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Internal Validation of the Investigator 26Plex QS Amplification Kit: a high-throughput multiplex assay for reference and low copy number DNA samples

2025-05Artigo de investigação Acesso restrito
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dc.contributor.authorNeves Cardoso, Paula Liliana
dc.contributor.authorSerra, Armando
dc.contributor.authorBogas, Vanessa
dc.contributor.authorBalsa, Filipa
dc.contributor.authorLopes, Virginia
dc.contributor.authorDario, Rita
dc.contributor.authorPorto, Maria João
dc.contributor.authorAmorim, António
dc.contributor.authorCorte Real, Francisco
dc.contributor.authorBrito, Pedro
dc.date.accessioned2026-01-22T14:30:16Z
dc.date.available2026-01-22T14:30:16Z
dc.date.issued2025-05
dc.description.abstractHuman singularity provides a foundation for forensic genetics, where the study of Short Tandem Repeats (STRs) is key. Furthermore, the development of polymerase chain reaction (PCR) has revolutionized the field by enabling the amplification of specific DNA markers. The Investigator® 26plex QS Amplification Kit is a highly robust multiplex PCR system, for human identification. In this study, we report the results obtained from the internal validation carried out at the Portuguese National Institute of Legal Medicine and Forensic Sciences. The results showed that the Investigator® 26plex QS kit proved to be a robust tool for the analysis of forensic samples, offering high sensitivity and specificity.eng
dc.identifier.citationCardoso, P., Serra, A., Bogas, V., Balsa, F., Lopes, V., Dario, R., Porto, M. J., Amorim, A., Corte Real, F., & Brito, P. (2025). Internal Validation of the Investigator 26Plex QS Amplification Kit: a high-throughput multiplex assay for reference and low copy number DNA samples. In . J. M. Butler, A. Carracedo, M. V. Lareu, A. Freire-Aradas, A. Mosquera-Miguel, C. Phillips, & M. de la Puente (Eds.) 30th Congress of the International Society for Forensic Genetics: Proceedings. (2nd rev. and corrected ed., pp. 1051–1059). Universidade de Santiago de Compostela. https://doi.org/10.15304/cc.2025.1869
dc.identifier.doihttps://dx.doi.org/10.15304/cc.2025.1869
dc.identifier.urihttp://hdl.handle.net/10400.26/61121
dc.language.isoeng
dc.peerreviewedyes
dc.publisherUniversidade de Santiago de Compostela
dc.relation.hasversionhttps://libros.usc.gal/en/categorias/1354-30th-congress-of-the-international-society-for-forensic-genetics-santiago-de-compostela-9-13-september-2024-339048-30th-congress-of-the-international-society-for-forensic-genetics.html#/29-formato-pdf/34-transaccion-gratis
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectValidation
dc.subjectAmplification Kit
dc.subjectThresholds
dc.subjectSTRs
dc.titleInternal Validation of the Investigator 26Plex QS Amplification Kit: a high-throughput multiplex assay for reference and low copy number DNA sampleseng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.endPage1059
oaire.citation.issue278
oaire.citation.startPage1051
oaire.citation.title30th Congress of the International Society for Forensic Genetics - Proceedings
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameNeves Cardoso
person.familyNameSerra
person.familyNameBogas
person.familyNameLopes
person.familyNameDario
person.familyNameAmorim
person.familyNameCorte Real Gonçalves
person.familyNameBrito
person.givenNamePaula Liliana
person.givenNameArmando
person.givenNameVanessa
person.givenNameVirginia
person.givenNameRita
person.givenNameAntónio
person.givenNameFrancisco
person.givenNamePedro
person.identifier.ciencia-id4814-A310-A09E
person.identifier.ciencia-idD71C-9C88-4E0E
person.identifier.ciencia-id0B18-918A-80ED
person.identifier.ciencia-id3E1A-80FE-9740
person.identifier.orcid0000-0003-0361-9768
person.identifier.orcid0000-0003-0162-9305
person.identifier.orcid0000-0001-7929-5814
person.identifier.orcid0000-0002-7915-826X
person.identifier.orcid0000-0001-6857-7840
person.identifier.orcid0000-0002-7506-4426
person.identifier.orcid0000-0003-1495-9362
person.identifier.orcid0000-0002-9622-8303
person.identifier.ridA-4414-2013
person.identifier.scopus-author-id35076952400
person.identifier.scopus-author-id35253426400
person.identifier.scopus-author-id6603582554
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