Repository logo
 
Publication

Assessment of the Cavidi ExaVir load assay for monitoring plasma viral load in HIV-2-infected patients

2017-08Journal article Restricted access
Simple item page
dc.contributor.authorBorrego, Pedro
dc.contributor.authorGonçalves, Maria Fátima
dc.contributor.authorGomes, Perpétua
dc.contributor.authorAraújo, Lavínia
dc.contributor.authorMoranguinho, Inês
dc.contributor.authorFigueiredo, Inês Brito
dc.contributor.authorBarahona, Isabel
dc.contributor.authorRocha, José
dc.contributor.authorMendonça, Claudino
dc.contributor.authorCruz, Maria Cesarina
dc.contributor.authorBarreto, Jorge
dc.contributor.authorTaveira, Nuno
dc.date.accessioned2019-12-12T14:29:51Z
dc.date.available2019-12-12T14:29:51Z
dc.date.issued2017-08
dc.description.abstractHIV plasma viral load is an established marker of disease progression and of response to antiretroviral therapy, but currently there is no commercial assay validated for the quantification of viral load in HIV-2-infected individuals. We sought to make the first clinical evaluation of Cavidi ExaVir Load (version 3) in HIV-2- infected patients. Samples were collected from a total of 102 individuals living in Cape Verde, and the HIV-2 viral load was quantified by both ExaVir Load and a reference in-house real-time quantitative PCR (qPCR) used in Portugal in 91 samples. The associations between viral load and clinical prognostic variables (CD4 T cell counts and antiretroviral therapy status) were similar for measurements obtained using ExaVir Load and qPCR. There was no difference between the two methods in the capacity to discriminate between nonquantifiable and quantifiable HIV-2 in the plasma. In samples with an HIV-2 viral load quantifiable by both methods (n 27), the measurements were highly correlated (Pearson r 0.908), but the ExaVir Load values were systematically higher relative to those determined by qPCR (median difference, 0.942 log10 copies/ml). A regression model was derived that enables the conversion of ExaVir Load results to those that would have been obtained by the reference qPCR. In conclusion, ExaVir Load version 3 is a reliable commercial assay to measure viral load in HIV-2-infected patients and therefore a valuable alternative to the inhouse assays in current use.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBorrego P, Gonçalves MF, Gomes P, Araújo L, Moranguinho I, Figueiredo IB, Barahona I, Rocha J, Mendonça C, Cruz MC, Barreto J, Taveira N. 2017. Assessment of the Cavidi ExaVir load assay for monitoring plasma viral load in HIV-2-infected patients. J Clin Microbiol 55:2367–2379. https://doi.org/10 .1128/JCM.00235-17.pt_PT
dc.identifier.doi10 .1128/JCM.00235-17pt_PT
dc.identifier.issn0095-1137
dc.identifier.issn1098-660X
dc.identifier.urihttp://hdl.handle.net/10400.26/30464
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relation.publisherversionhttps://doi.org/10.1128/JCM.00235-17pt_PT
dc.subjectCape Verdept_PT
dc.subjectCavidi ExaVir loadpt_PT
dc.subjectHIV-2pt_PT
dc.subjectResource-limited settingspt_PT
dc.subjectViral load assaypt_PT
dc.titleAssessment of the Cavidi ExaVir load assay for monitoring plasma viral load in HIV-2-infected patientspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2379pt_PT
oaire.citation.startPage2367pt_PT
oaire.citation.titleJournal of Clinical Microbiologypt_PT
oaire.citation.volume55(8)pt_PT
rcaap.embargofctPolítica de copyright do editorpt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Artigo_NTaveira_2019_04_Final.pdf
Size:
709.05 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

EM - Artigos Científicos