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Evaluation of hair hydrolysis and MEPS columns for the detection of amphetamines derivatives in hair

2023-10Other Open access
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dc.contributor.authorPires, Bruno
dc.contributor.authorSimão, Ana Y.
dc.contributor.authorRosado, Tiago
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorBarroso, Mário
dc.date.accessioned2024-01-25T13:55:55Z
dc.date.available2024-01-25T13:55:55Z
dc.date.issued2023-10
dc.descriptionComunicação apresentada no 10º Congresso Iberoamericano de Ciências Farmacêuticas, Coimbra, Outubro de 2023. Abstract da comunicação publicado na Acta Farmacêutica Portuguesa. vol. de Outubro 2023. ISSN 2182-3340.pt_PT
dc.description.abstractIntroduction: Different extraction and cleanup procedures have been used to identify amphetamines in hair samples. The microextraction by packed sorbent (MEPS), a miniaturized approach of the classic solid-phase extraction (SPE), has only been used so far to determine the presence of amphetamine (AMP) and methamphetamine (MAMP) in hair. The aim of this work was to evaluate different hair sample extraction conditions, namely hydrolysis, to maximize the detection of AMP, MAMP, MDA, MDMA, MDE, and MBDB in combination with MEPS and GC-MS. Methodology: Pulverized authentic hair (50mg) was incubated with 500μL of NaOH 1M at 80ºC for 1H, 60ºC overnight and 45ºC overnight1. After incubation, the extracts were neutralized with 50μL of 10M HCl and subsequently centrifuged. The supernatant was tested with several conditions to access the most suitable when paired with MEPS clean-up. The studied conditions were as follows: the addition of 500μL of H2O; supernatant filtration followed by addition of H2O; and precipitation with cold ACN followed by centrifugation. After clean-up, 30μL of MBTFA were added for evaporation under nitrogen gas. After being fully dried, 50μL of MBTFA was added and derivatization was done with a microwave oven. Finally, the extract was injected into a GC-MS and analysed. Results: Results obtained from these tests indicated that the best hydrolysis for this purpose was obtained with 500μL NaOH 1M at 45ºC overnight, with the addition of H2O before MEPS clean-up. The clean-up procedure was previously optimized by design of experiments (not being the scope of this study). Furthermore, M1 and C18 MEPS sorbents were tested to check which one would provide cleaner extracts and greater analyte signals. A concentration of 2.00 ng/mg of AMP, MAMP, MDA, MDMA, MDE, and MBDB was used for this test. Discussion: Considering the results, both columns presented similar recoveries for each amphetamine, when using equal hydrolysis and MEPS conditions.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/49364
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationHair as a monitoring tool for new drugs: synthetic cannabinoids and opioids
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHair analysispt_PT
dc.subjectAmphetaminespt_PT
dc.subjectHydrolysispt_PT
dc.titleEvaluation of hair hydrolysis and MEPS columns for the detection of amphetamines derivatives in hairpt_PT
dc.typeother
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHair as a monitoring tool for new drugs: synthetic cannabinoids and opioids
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PDQI_CENTRO/2020.09070.BD/PT
oaire.citation.conferencePlaceCoimbrapt_PT
oaire.citation.title10º Congresso Iberoamericano de Ciências Farmacêuticaspt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPDQI_CENTRO
person.familyNameBarroso
person.givenNameMário
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeotherpt_PT
relation.isAuthorOfPublication294b555e-48c4-4013-b981-b8917400c56c
relation.isAuthorOfPublication.latestForDiscovery294b555e-48c4-4013-b981-b8917400c56c
relation.isProjectOfPublication4ca3c3c9-1802-4c7f-8730-9547236896fe
relation.isProjectOfPublication56ce2e5c-35e5-4eb7-8152-2e87e77f638c
relation.isProjectOfPublicatione951cc6d-0466-4e2e-b4d9-7dd14ba7a196
relation.isProjectOfPublication.latestForDiscovery4ca3c3c9-1802-4c7f-8730-9547236896fe

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