How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry : a tutorial

The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.

Palavras-chave

Immuno-mass spectrometry Viral detection MALDI-TOF-MS LC-ESI-Q-TOF-MS/MS

URI

http://hdl.handle.net/10400.26/61937

Citação

Hugo M. Santos, Luís B. Carvalho, Carlos Lodeiro, Gonçalo Martins, Inês L. Gomes, Wilson D.T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, António P.A. Matos, J.L. Capelo, How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial, Microchemical Journal, Volume 186, 2023, 108323, https://doi.org/10.1016/j.microc.2022.108323

Editora

Elsevier

DOI

10.1016/j.microc.2022.108323

Coleções

EM - Artigos Científicos

Licença CC

cclicense-by-nc-nd

Métricas Alternativas

Ver registo completo