Publicação
The influence of genetic polymorphisms on the expression of interleukin-1beta, prostaglandin E2 and tumor necrosis factor alpha in peri-implant crevicular fluid : a cross-sectional study
| datacite.subject.fos | Ciências Médicas::Ciências da Saúde | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Cardoso, José Maria | |
| dc.contributor.author | Ribeiro, Ana Clara | |
| dc.contributor.author | Botelho, João | |
| dc.contributor.author | Proença, Luís | |
| dc.contributor.author | Noronha, Susana | |
| dc.contributor.author | Alves, Ricardo Castro | |
| dc.date.accessioned | 2026-04-27T08:39:06Z | |
| dc.date.available | 2026-04-27T08:39:06Z | |
| dc.date.issued | 2024-01 | |
| dc.description.abstract | The aim of this study was to evaluate the possible relationships between polymorphisms in the interleukin-1 (IL-1) A, IL-1B, and IL-1RN genes and concentrations of the inflammatory mediators IL-1β, tumor necrosis factor-alpha (TNF-α), and prostaglandin E2 (PGE2) in peri-implant crevicular fluid (PICF). A cross-sectional analytical study was conducted on 51 patients with dental implants. Samples from the buccal mucosa were obtained, and genetic analysis was performed using the real-time polymerase chain reaction (PCR) technique for IL-1A and IL-1B and PCR and restriction fragment length polymorphism analysis for IL-1RN. For the biochemical analysis, the concentrations of IL-1β and TNF-α were analyzed using multiplexed fluorescent sphere immunoassays, and PGE2 by enzyme-linked immunosorbent assay. In patients with detected IL-1RN polymorphism, there was an increase in the concentration of the three mediators with statistically significant differences in the mean values of TNF-α and PGE2, regardless of peri-implant health status (p = 0.002 and p = 0.049, respectively). The concentrations of all three mediators were positively and significantly correlated (IL-1β vs. TNF-α Rho = 0.480, p < 0.001; IL-1β vs. PGE2 Rho = 0.382, p = 0.006; and TNF-α vs. PGE2 Rho = 0.528, p < 0.001). We can conclude that the IL-1RN polymorphism exerts an influence on the PICF immune response, which may explain the influence of this genetic polymorphism on the occurrence of peri-implantitis. | eng |
| dc.identifier.citation | Cardoso JM, Ribeiro AC, Botelho J, Proença L, Noronha S, Alves RC. The Influence of Genetic Polymorphisms on the Expression of Interleukin-1beta, Prostaglandin E2 and Tumor Necrosis Factor Alpha in Peri-Implant Crevicular Fluid: A Cross-Sectional Study. International Journal of Molecular Sciences. 2024; 25(1):651. https://doi.org/10.3390/ijms25010651 | |
| dc.identifier.doi | 10.3390/ijms25010651 | |
| dc.identifier.issn | 1422-0067 | |
| dc.identifier.uri | http://hdl.handle.net/10400.26/62866 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI | |
| dc.relation.hasversion | https://doi.org/10.3390/ijms25010651 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | dental implants | |
| dc.subject | genotype | |
| dc.subject | interleukin-1 | |
| dc.subject | interleukin-1alpha | |
| dc.subject | peri-implantitis | |
| dc.subject | polymorphisms | |
| dc.title | The influence of genetic polymorphisms on the expression of interleukin-1beta, prostaglandin E2 and tumor necrosis factor alpha in peri-implant crevicular fluid : a cross-sectional study | eng |
| dc.type | contribution to journal | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 1 | |
| oaire.citation.startPage | 651 | |
| oaire.citation.title | International Journal of Molecular Sciences | |
| oaire.citation.volume | 25 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |