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Influência dos genes dos sistemas dopaminérgico e serotoninérgico no comportamento agressivo numa população universitária
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Advisor(s)
Abstract(s)
Vários estudos têm demonstrado que fatores genéticos e ambientais, cerca de 50% está relacionado com influências genéticas e os restantes 50% explicados por fatores ambientais não compartilhados com nenhum membro da família, influenciam uma grande variedade dos comportamentos humanos, incluindo o comportamento agressivo. A genética comportamental têm tentado correlacionar e compreender a base da genética da agressividade. Genes relacionados com serotonina (5-HT) e dopamina (DA) são os mais relacionados com a agressividade.
Este trabalho está inserido num projeto que visa a estudar a ‘’Influência dos genes no comportamento agressivo numa população universitária’’, assim tivemos apenas como objetivo a caracterização genotípica de apenas dois genes (MAO-A e SLC6A4) de entre outros genes todos eles descritos na literatura como associados à agressão humana.
A amostra para a caracterização genotípica inclui trinta e três jovens estudantes universitários com idades entre 17 e 31 anos. Os polimorfismos a estudar foram: VNTR 30pb do gene MAO-A e 5-HTTLPR e rs25531 do gene SLC6A4. Os genótipos dos indivíduos amostrados foram identificados por PCR ou PCR-RFLP.
Relativamente ao polimorfismo VNTR 30pb do gene MAO-A, revelou-se nove indivíduos homozigóticos para o alelo 4 (4R), treze indivíduos heterozigóticos para os alelos 4 (4R) e 3 (3R), dois indivíduos heterozigótico para os alelos 3 (3R) e 5 (5R), três indivíduos homozigóticos para o alelo 3 (3R) e três indivíduos homozigóticos para o alelo 5 (5R). Observamos mais indivíduos com alelos MAO-A-H do que para os alelos MAO-A-L, pois segundo a literatura os alelos com 3(3R) e 4(4R) repetições são os mais comuns na espécie humana. Em relação ao polimorfismo 5-HTTLPR do gene SLC6A4 revelou-se a presença de seis indivíduos heterozigóticos (L/S), um indivíduo homozigótico (S/S) e quatro indivíduos homozigóticos (L/L). O polimorfismo rs25531 do gene SLC6A4 apenas conseguiu-se genotipar apenas quatro indivíduo: Um indivíduo homozigótico para o alelo SS, dois indivíduos heterozigóticos para o alelo LaS e um individuo homozigótico LaLa.
Several studies have shown that genetic and environmental factors influence a wide variety of human behaviours, including aggressive behaviour. About 50% of these factors are related to genetic influences and the remaining 50% are explained by environmental factors not shared with any family member. Behavioural genetics have attempted to correlate and understand the basis of the genetics of aggression. Serotonin (5-HT) and dopamine (DA) related genes are the most related to aggressiveness. This work is part of a project aiming to study the ''Influence of genes on aggressive behaviour in a university population'' and it was our purpose to start with the genotypic characterisation of only two genes (MAO-A and SLC6A4) among other genes all described in the literature as associated with human aggression. The sample for the genotypic characterisation included thirty-three young university students aged between 17 and 31 years. The polymorphisms to be studied were: VNTR 30pb of MAO-A gene ,5-HTTLPR and rs25531 of SLC6A4 gene. The genotypes of the sampled individuals were identified by PCR or PCR-RFLP molecular biology techniques. Regarding the 30bp VNTR polymorphism of the MAO-A gene, nine homozygote individuals carrying the 4 (4R) allele were identified, thirteen heterozygote individuals with the 4 (4R) and 3 (3R) alleles, two heterozygote individuals with the 3 (3R) and 5 (5R) alleles, three homozygote individuals with the 3 (3R) allele and three homozygote individuals with the 5 (5R) allele. We observed more individuals with MAO-A-H alleles than for MAO-A-L alleles, because according to the literature the alleles with 3(3R) and 4(4R) repeats are the most common in the human species. Regarding the 5-HTTLPR polymorphism of the SLC6A4 gene we obtained the following results: six heterozygotic individuals (L/S), one homozygotic individual (S/S) and four homozygotic individuals (L/L). With the rs25531 polymorphism of the SLC6A4 gene it was only possible to genotype four individuals: one homozigotic for the SS allele, two heterozygotes for the LaS allele and one homozygote LaLa.
Several studies have shown that genetic and environmental factors influence a wide variety of human behaviours, including aggressive behaviour. About 50% of these factors are related to genetic influences and the remaining 50% are explained by environmental factors not shared with any family member. Behavioural genetics have attempted to correlate and understand the basis of the genetics of aggression. Serotonin (5-HT) and dopamine (DA) related genes are the most related to aggressiveness. This work is part of a project aiming to study the ''Influence of genes on aggressive behaviour in a university population'' and it was our purpose to start with the genotypic characterisation of only two genes (MAO-A and SLC6A4) among other genes all described in the literature as associated with human aggression. The sample for the genotypic characterisation included thirty-three young university students aged between 17 and 31 years. The polymorphisms to be studied were: VNTR 30pb of MAO-A gene ,5-HTTLPR and rs25531 of SLC6A4 gene. The genotypes of the sampled individuals were identified by PCR or PCR-RFLP molecular biology techniques. Regarding the 30bp VNTR polymorphism of the MAO-A gene, nine homozygote individuals carrying the 4 (4R) allele were identified, thirteen heterozygote individuals with the 4 (4R) and 3 (3R) alleles, two heterozygote individuals with the 3 (3R) and 5 (5R) alleles, three homozygote individuals with the 3 (3R) allele and three homozygote individuals with the 5 (5R) allele. We observed more individuals with MAO-A-H alleles than for MAO-A-L alleles, because according to the literature the alleles with 3(3R) and 4(4R) repeats are the most common in the human species. Regarding the 5-HTTLPR polymorphism of the SLC6A4 gene we obtained the following results: six heterozygotic individuals (L/S), one homozygotic individual (S/S) and four homozygotic individuals (L/L). With the rs25531 polymorphism of the SLC6A4 gene it was only possible to genotype four individuals: one homozigotic for the SS allele, two heterozygotes for the LaS allele and one homozygote LaLa.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Agressividade MAO-A SLC6A4 Comportamento agressivo Polimorfismos Serotonina