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HIV-1 diversity and pre-treatment drug resistance in the era of integrase inhibitor among newly diagnosed ART-naïve adult patients in Luanda, Angola

2024-07Contributo em revista Acesso aberto
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datacite.subject.fosCiências Médicas::Ciências da Saúde
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorSebastião, Cruz S.
dc.contributor.authorAbecasis, Ana B.
dc.contributor.authorJandondo, Domingos
dc.contributor.authorSebastião, Joana M. K.
dc.contributor.authorVigário, João
dc.contributor.authorComandante, Felícia
dc.contributor.authorPingarilho, Marta
dc.contributor.authorPocongo, Bárbara
dc.contributor.authorCassinela, Edson
dc.contributor.authorGonçalves, Fátima
dc.contributor.authorGomes, Perpétua
dc.contributor.authorGiovanetti, Marta
dc.contributor.authorFrancisco, Ngiambudulu M.
dc.contributor.authorSacomboio, Euclides
dc.contributor.authorBrito, Miguel
dc.contributor.authorVasconcelos, Jocelyne Neto de
dc.contributor.authorMorais, Joana
dc.contributor.authorPimentel, Victor
dc.date.accessioned2026-04-02T08:34:35Z
dc.date.available2026-04-02T08:34:35Z
dc.date.issued2024-07
dc.description.abstractThe surveillance of drug resistance in the HIV-1 naïve population remains critical to optimizing the effectiveness of antiretroviral therapy (ART), mainly in the era of integrase strand transfer inhibitor (INSTI) regimens. Currently, there is no data regarding resistance to INSTI in Angola since Dolutegravir-DTG was included in the first-line ART regimen. Herein, we investigated the HIV-1 genetic diversity and pretreatment drug resistance (PDR) profile against nucleoside/tide reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors (PIs), and INSTIs, using a next-generation sequencing (NGS) approach with MinION, established to track and survey DRMs in Angola. This was a cross-sectional study comprising 48 newly HIV-diagnosed patients from Luanda, Angola, screened between March 2022 and May 2023. PR, RT, and IN fragments were sequenced for drug resistance and molecular transmission cluster analysis. A total of 45 out of the 48 plasma samples were successfully sequenced. Of these, 10/45 (22.2%) presented PDR to PIs/NRTIs/NNRTIs. Major mutations for NRTIs (2.2%), NNRTIs (20%), PIs (2.2%), and accessory mutations against INSTIs (13.3%) were detected. No major mutations against INSTIs were detected. M41L (2%) and I85V (2%) mutations were detected for NRTI and PI, respectively. K103N (7%), Y181C (7%), and K101E (7%) mutations were frequently observed in NNRTI. The L74M (9%) accessory mutation was frequently observed in the INSTI class. HIV-1 pure subtypes C (33%), F1 (17%), G (15%), A1 (10%), H (6%), and D (4%), CRF01_AG (4%) were observed, while about 10% were recombinant strains. About 31% of detected HIV-1C sequences were in clusters, suggesting small-scale local transmission chains. No major mutations against integrase inhibitors were detected, supporting the continued use of INSTI in the country. Further studies assessing the HIV-1 epidemiology in the era of INSTI-based ART regimens are needed in Angola.eng
dc.identifier.citationSebastião, C.S., Abecasis, A.B., Jandondo, D. et al. HIV-1 diversity and pre-treatment drug resistance in the era of integrase inhibitor among newly diagnosed ART-naïve adult patients in Luanda, Angola. Sci Rep 14, 15893 (2024). https://doi.org/10.1038/s41598-024-66905-1
dc.identifier.doi10.1038/s41598-024-66905-1
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.26/62579
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Nature
dc.relation.hasversionhttps://doi.org/10.1038/s41598-024-66905-1
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHIV-1
dc.subjectGenetic diversity
dc.subjectDrug resistance mutations
dc.subjectINSTI
dc.subjectNGS
dc.subjectAngola
dc.titleHIV-1 diversity and pre-treatment drug resistance in the era of integrase inhibitor among newly diagnosed ART-naïve adult patients in Luanda, Angolaeng
dc.typecontribution to journal
dspace.entity.typePublication
oaire.citation.startPage15893
oaire.citation.titleScientific Reports
oaire.citation.volume14
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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