Publication
The Determination of Cannabinoids in Urine Samples Using Microextraction by Packed Sorbent and Gas Chromatography-Mass Spectrometry
| dc.contributor.author | Rosendo, Luana M. | |
| dc.contributor.author | Rosado, Tiago | |
| dc.contributor.author | Oliveira, Patrik | |
| dc.contributor.author | Simão, Ana Y. | |
| dc.contributor.author | Margalho, Cláudia | |
| dc.contributor.author | Costa, Suzel | |
| dc.contributor.author | Passarinha, Luís A. | |
| dc.contributor.author | Barroso, Mário | |
| dc.contributor.author | Gallardo, Eugenia | |
| dc.date.accessioned | 2022-09-26T11:21:27Z | |
| dc.date.available | 2022-09-26T11:21:27Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Cannabis is the most consumed illicit drug worldwide, and its legal status is a source of concern. This study proposes a rapid procedure for the simultaneous quantification of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH), cannabidiol (CBD), and cannabinol (CBN) in urine samples. Microextraction by packed sorbent (MEPS) was used to pre-concentrate the analytes, which were detected by gas chromatography–mass spectrometry. The procedure was previously optimized, and the final conditions were: conditioning with 50 µL methanol and 50 µL of water, sample load with two draw–eject cycles, and washing with 310 µL of 0.1% formic acid in water with 5% isopropanol; the elution was made with 35 µL of 0.1% ammonium hydroxide in methanol. This fast extraction procedure allowed quantification in the ranges of 1–400 ng/mL for THC and CBD, 5–400 ng/mL for CBN and 11-OH-THC, and 10–400 ng/mL for THC-COOH with coefficients of determination higher than 0.99. The limits of quantification and detection were between 1 and 10 ng/mL using 0.25 mL of sample. The extraction efficiencies varied between 26 and 85%. This analytical method is the first allowing the for determination of cannabinoids in urine samples using MEPS, a fast, simple, and low-cost alternative to conventional techniques | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Rosendo, L. M., Rosado, T., Oliveira, P., Simão, A. Y., Margalho, C., Costa, S., Passarinha, L. A., et al. (2022). The Determination of Cannabinoids in Urine Samples Using Microextraction by Packed Sorbent and Gas Chromatography-Mass Spectrometry. Molecules, 27(17), 5503 | pt_PT |
| dc.identifier.doi | 10.3390/molecules27175503 | pt_PT |
| dc.identifier.issn | 1420-3049 | |
| dc.identifier.uri | http://hdl.handle.net/10400.26/41844 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | Health Sciences Research Centre | |
| dc.relation | Applied Molecular Biosciences Unit | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Cannabinoids | pt_PT |
| dc.subject | Tetrahydrocannabinol | pt_PT |
| dc.subject | Urine | pt_PT |
| dc.subject | Gas Chromatography-mass Spectrometry | pt_PT |
| dc.title | The Determination of Cannabinoids in Urine Samples Using Microextraction by Packed Sorbent and Gas Chromatography-Mass Spectrometry | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Health Sciences Research Centre | |
| oaire.awardTitle | Applied Molecular Biosciences Unit | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT | |
| oaire.citation.conferencePlace | BASEL, SWITZERLAND | pt_PT |
| oaire.citation.issue | 17 | pt_PT |
| oaire.citation.startPage | 5503 | pt_PT |
| oaire.citation.title | Molecules | pt_PT |
| oaire.citation.volume | 27 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| person.familyName | Margalho | |
| person.familyName | Barroso | |
| person.givenName | Cláudia | |
| person.givenName | Mário | |
| person.identifier.ciencia-id | A211-521D-90CA | |
| person.identifier.ciencia-id | 5110-F476-ECCC | |
| person.identifier.orcid | 0000-0003-1661-0367 | |
| person.identifier.orcid | 0000-0001-8848-2734 | |
| person.identifier.rid | KII-2916-2024 | |
| person.identifier.scopus-author-id | 8655072100 | |
| person.identifier.scopus-author-id | 35329243100 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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