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The role of DNA concentrations in forensic casework results : regression models application

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dc.contributor.authorVieira Da Silva, Cláudia
dc.contributor.authorAmorim, António
dc.contributor.authorAfonso Costa, Heloísa
dc.contributor.authorPorto, Maria João
dc.contributor.authorCorte Real, F.
dc.contributor.authorAntunes, Marilia
dc.date.accessioned2022-04-04T14:48:10Z
dc.date.available2022-04-04T14:48:10Z
dc.date.issued2019
dc.descriptionPóster apresentado no 28th Congress of International Society For Forensic Genetics (ISFG 2019), Praga, República Checa, 9-13 de Setembro de 2019pt_PT
dc.description.abstractIn forensic DNA typing, short tandem repeats (STRs) are the most frequently genotyped markers in order to distinguish between individuals and to relate them to a crime or to exonerate the innocent. In recent years, new controversies have arisen with the advent of more sensitive techniques, allowing profiles to be recovered from minimum amounts of DNA, hence, bringing challenges to weight of evidence evaluation for forensic DNA profiles obtained from low template DNA samples. Introduction of interpretation models, or even new weight of evidence software should be accompanied by a measure of uncertainty that is part of any biological analysis. Specially, due to stochastic effects, the reliability of the obtained profiles might differ between machinery, workflow and also PCR settings in use in different laboratories. In this work we try to understand the relation between Peak Area, DNA concentration and also size marker, using adequate regression models. Buccal swabs from 180 individuals, with unknown identity, were selected for this study. DNA was extracted with prep-n-go™ buffer and quantified using Quantifiler® Trio DNA Quantification kit in a 7500 Real-Time PCR System (Applied Biosystems). STR amplification was performed with Powerplex Fusion 6C amplification kit (Promega). Amplified PCR products were separated and detected in an Applied Biosystems® 3500 Genetic Analyzer using manufacturer’s conditions. Electrophoresis results were analysed with GeneMapper® ID-X v1.4. Statistical analysis was performed with R Studio. Our results allow having an important overview about the relation between DNA concentrations, peak area, and size of the studied genetic markers.
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/40048
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectDNA Concentrationspt_PT
dc.subjectForensic DNApt_PT
dc.subjectRegression Modelspt_PT
dc.subjectStatistical modelspt_PT
dc.titleThe role of DNA concentrations in forensic casework results : regression models applicationpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePraga, República Checapt_PT
oaire.citation.title28th Congress of International Society For Forensic Geneticspt_PT
person.familyNameda Silva
person.familyNameAmorim
person.familyNameAfonso Costa
person.familyNameCorte Real Gonçalves
person.familyNamede Sousa Antunes
person.givenNameCláudia
person.givenNameAntónio
person.givenNameHeloísa
person.givenNameFrancisco
person.givenNameMarilia Cristina
person.identifierG-8864-2015
person.identifier.ciencia-idD218-0D74-678B
person.identifier.ciencia-id0B18-918A-80ED
person.identifier.ciencia-id1A16-4A6A-A8F9
person.identifier.orcid0000-0003-0373-7457
person.identifier.orcid0000-0002-7506-4426
person.identifier.orcid0000-0002-7823-7687
person.identifier.orcid0000-0003-1495-9362
person.identifier.orcid0000-0002-1257-2829
person.identifier.ridA-4414-2013
person.identifier.ridJ-5725-2015
person.identifier.scopus-author-id9839086200
person.identifier.scopus-author-id35253426400
person.identifier.scopus-author-id24729171700
person.identifier.scopus-author-id6603582554
person.identifier.scopus-author-id16300632900
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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