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Polyhydroxyalkanoates: waste glycerol upgrade into electrospunfibrous scaffolds for stem cells culture

2014-11Journal article Restricted access
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dc.contributor.authorCanadas, Raphaël F.
dc.contributor.authorCavalheiro, João M.B.T.
dc.contributor.authorGuerreiro, João D.T.
dc.contributor.authorAlmeida, M. Catarina M.D. de
dc.contributor.authorPollet, Eric
dc.contributor.authorSilva, Cláudia Lobato da
dc.contributor.authorFonseca, M.M.R. da
dc.contributor.authorFerreira, Frederico Castelo
dc.date.accessioned2017-08-07T11:27:06Z
dc.date.available2017-08-07T11:27:06Z
dc.date.issued2014-11
dc.description.abstract"This integrated study shows that waste glycerol can be bio-valorized by the fabrication of electrospun scaffolds for stem cells. Human mesenchymal stem cells (hMSC) provide an interesting model of regenerating cells because of their ability to differentiate into osteo-, chrondro-, adipo- and myogenic lineages. Moreover, hMSC have modulatory properties with potential on treatment of immunologic diseases. Electrospun fiber meshes offer tunable mechanical and physical properties that can mimic the structure of the native extracellular matrix, the natural environment where cells inhabit. Following a biorefinery approach, crude glycerol directly recovered from a biodiesel post-reaction stream was fed as major C source to Cupriavidus necator DSM 545 to produce polyhydroxyalkanoates at polymer titers of 9–25 g/L. Two of the P(3HB-4HB-3HV) terpolymers produced, one containing 11.4% 4HB and 3.5% 3HV and the other containing 35.6% 4HB and 3.4% 3HV, were electrospun into fibers of average diameters of 600 and 1400 nm, respectively. hMSC were cultured for 7 days in both fiber meshes, showing their ability to support stem cell growth at acceptable proliferation levels. Comparative results clearly demonstrate that scaffold topology is critical, with electrospun PHA fibers succeeding on the support of significant cell adhesion and proliferation, where planar PHA films failed."pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRaphaël F. Canadas, João M.B.T. Cavalheiro, João D.T. Guerreiro, M. Catarina M.D. de Almeida, Eric Pollet, Cláudia Lobato da Silva, M.M.R. da Fonseca, Frederico Castelo Ferreira, Polyhydroxyalkanoates: Waste glycerol upgrade into electrospun fibrous scaffolds for stem cells culture, International Journal of Biological Macromolecules, Volume 71, 2014, Pages 131-140, ISSN 0141-8130, http://dx.doi.org/10.1016/j.ijbiomac.2014.05.008.pt_PT
dc.identifier.doi10.1016/j.ijbiomac.2014.05.008pt_PT
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/10400.26/18768
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationScalable Reactor Systems for the Production of Human Mesenchymal Stem Cells
dc.relationPRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
dc.relation.publisherversionhttps://doi.org/10.1016/j.ijbiomac.2014.05.008pt_PT
dc.subjectPolyhydroxyalkanoatespt_PT
dc.subjectWaste glycerolpt_PT
dc.subjectStem cell scaffoldspt_PT
dc.subjectElectrospinningpt_PT
dc.subjectMesenchymal stem cellapt_PT
dc.titlePolyhydroxyalkanoates: waste glycerol upgrade into electrospunfibrous scaffolds for stem cells culturept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleScalable Reactor Systems for the Production of Human Mesenchymal Stem Cells
oaire.awardTitlePRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEQU-EQU%2F114231%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F45266%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F26678%2F2006/PT
oaire.citation.endPage140pt_PT
oaire.citation.startPage131pt_PT
oaire.citation.titleInternational Journal of Biological Macromoleculespt_PT
oaire.citation.volume71pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica de copyright do editorpt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication60cadb21-d912-43c4-88a8-94a76fca706b
relation.isProjectOfPublication8dda2e28-74d4-44ba-9eda-84aaf3a6eb0d
relation.isProjectOfPublication54143930-0087-45be-ae76-b932ef0f1af1
relation.isProjectOfPublication.latestForDiscovery60cadb21-d912-43c4-88a8-94a76fca706b

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