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- Genetic Data of Four X-Chromosomal STRs in a Population Sample of Santa Catarina, BrazilPublication . Cainé, Laura; Pontes, Lurdes; Abrantes, David; Lima, Gabriela; Pinheiro, FátimaA total of 184 healthy unrelated individuals (70 females and 114 males), autochthonous from Santa Catarina, Brazil.
- Allele frequencies of sixteen STRs in the population of Northern PortugalPublication . Pinheiro, M.F.; Cainé, Laura; Pontes, Lurdes; Abrantes, David; Lima, Gabriela; Pereira, Maria J.; Rezende, PedroAllele frequencies of sixteen autossomal short tandem repeats (STRs), D3S1358, VWA, D16S539, D8S1179, D21S11, D18S51, TH01, FGA, D5S818, D13S317, D7S820, TPOX, CSF1PO, Penta D, Penta E (included in the PowerPlex 16 kit), and the SE33 (PowerPlex ES Monoplex System SE33) were determined in a sample of 200 healthy unrelated individuals from the north of Portugal.
- Genetic Data of 4 X-Chromosomal Short Tandem Repeats in a North of Portugal PopulationPublication . Cainé, Laura; Pontes, Lurdes; Abrantes, David; Lima, Gabriela; Pinheiro, FátimaOne hundred unrelated females and 100 unrelated males, autochthonous, healthy, from the North of Portugal.
- Genetic Data of Five STR Loci in a Population Sample of Santa Catarina, BrazilPublication . Pinheiro, Fátima; Lima, Gabriela; Abrantes, David; Pontes, Lurdes; Cainé, LauraIn routine forensic casework, it is important to establish a population database for further reliable statistical analysis.
- Allele Frequencies of 15 Loci Using AmpFℓSTR Identifiler Kit in a Northern Portuguese PopulationPublication . Abrantes, David; Lima, Gabriela; Cainé, Laura; Pontes, Lurdes; Pinheiro, Fátima250 unrelated and healthy individuals from Portugal.
- Assessment of DNA and mtDNA Degradation in Sperm Cells Collected by Laser Micro-dissectionPublication . Lima, Gabriela; Correia de Sá, P; Porto, MJ; Cainé, Laura; Costa, SSexual assault samples are among the most frequently analyzed in a forensic laboratory. These accounts for almost half of all samples processed routinely and a large portion of these cases remain unsolved. These samples often pose problems to traditional analytic methods of identification because they consist most frequently of cell mixtures from at least two contributors: the victim (usually female) and the perpetrator (usually male). In this study we prepared simulated sexual assault samples and conducted amplification and DNA genotyping from sperm cells collected with laser microdissection at different time intervals to assess the DNA degradation at those intervals. Furthermore, we then investigated the possibility of recovering mtDNA from the collected sperm cells, particularly in cases where autosomal DNA was not suitably amplified. With this work we determined that it becomes possible to extend the timeframe for performing an analysis, by researching other sources of DNA, namely mtDNA.
- Allele frequencies and population data for 17 Y-STR loci (AmpFℓSTR® Y-filer™) in a Northern Portuguese population samplePublication . Pontes, Lurdes; Cainé, Laura; Abrantes, David; Lima, Gabriela; Pinheiro, Maria de FátimaAllele frequencies and population data for 17 Y-STR loci included in a new commercial kit that has recently been available, the AmpFlSTR Y-filer PCR amplification kit (Applied Biosystems), that permits the simultaneous amplification of all the markers included in the actually used European "extended haplotype", DYS19, DYS189I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385I/II, DYS438, DYS439 and also DYS437, DYS448, DYS456, DYS458, DYS635 and Y GATA H4, were obtained from a sample of 175 healthy unrelated males and 45 father-son pairs from the North of Portugal. A total of 171 haplotypes were identified, of which 167 were unique and 4 were found in 2 individuals. The haplotype diversity (99.97%) and discrimination capacity (95.43%) were calculated. We report some non-standard situations, such as allele duplications and mutations. We also report a case of disputed paternity in which duplicated alleles plus an inconsistency of the transmitted alleles appeared.
- DNA typing of Diptera collected from human corpses in PortugalPublication . Cainé, Laura; Corte Real, Francisco; Saloña-Bordas, Marta I.; Martínez de Pancorbo, M.; Lima, Gabriela; Magalhães, Teresa; Pinheiro, FátimaMedico-legal entomology, one area in the broad field of entomology, is routinely used in forensic applications. Insects are often collected from a corpse during criminal information related to the body, but requires the fast and accurate identification of the species attracted to the remains. The local entomofauna in most cases is important for explaining entomological evidence. The survey of the local entomofauna has become a fundamental first step in forensic entomological studies, because different geographical distributions, seasonal and environmental factors may influence the decomposition process and the occurrence of different species on corpses. A morphological and DNA-based methods for species identification were used in this study. Thirty-two cases are reported from indoors and outdoors conditions. Specimens were collected from corpses during autopsy procedures in the National Institute of Legal Medicine, Portugal, and cases were summarized by sex, death local, month of discovery, probable cause of death, species found and number of analyzed specimens. Just eight species, mainly Calliphoridae together with one Sarcophagidae were reported from corpses. The DNA sequencing was performed to study the mitochondrial encoded subunit I of the cytochrome oxidase gene. The aim of this work was the beginning of a database of the cadaveric entomofauna in Portugal.
- Genetic Data of 11 Y-Chromosome STRs in Males from a North of Portugal PopulationPublication . Pinheiro, Fátima; Lima, Gabriela; Abrantes, David; Cainé, Laura; Pontes, LurdesData show that the haplotype is highly polymorphic and discriminative in the North of Portugal population.
- Genetic Data of Eight Y-Chromosome STRs in Males from Santa Catarina, BrazilPublication . Pinheiro, Fátima; Lima, Gabriela; Abrantes, David; Pontes, Lurdes; Cainé, Laura94 different haplotypes were observed, 86 of them being unique.