Browsing by Issue Date, starting with "2018-05-02"
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- Gestão da segurança e qualidade alimentar numa empresa de congeladosPublication . Antunes, Joana Patrícia Marques; Gândara, João Filipe MarquesResumo Este relatório resulta do estágio do Mestrado em Engenharia Alimentar da Escola Superior Agrária de Coimbra. O estágio decorreu na empresa Frijobel, S.A., com sede em Espinheiro, Penela, entre os dias 2 de janeiro e 2 de julho de 2017. Os temas centrais do trabalho desenvolvido são a segurança e a qualidade alimentar, aspetos fundamentais quer para o consumidor final quer para os parceiros comerciais. A gestão da qualidade e segurança alimentar deve ser feita de forma sistemática, pela implementação de um sistema de gestão estruturado e integrado nas atividades globais de gestão da organização. Existem vários referenciais normativos para estes sistemas de gestão, sendo que no estágio foram implementados os requisitos do referencial BRC Food, versão 7, uma vez que existia o interesse da Frijobel em obter a certificação por esse referencial. Assim, o principal objectivo deste estágio foi a implementação, na Frijobel, de um sistema de gestão da qualidade e segurança alimentar que satisfaça os requisitos do referencial BRC. Para isso foi necessário: ✤ O desenvolvimento do suporte documental do sistema de gestão, tendo em vista a sua adequação e posterior controlo; ✤ A revisão da documentação técnica já existente e a criação da documentação em falta; ✤ A criação de procedimentos de monitorização e controlo. Os objectivos propostos para o estágio foram alcançados, tendo a Frijobel conseguido, em 20 de junho de 2017, a certificação pela norma BRC. Palavras-chave: segurança alimentar, qualidade alimentar, sistemas de gestão, BRC Food versão 7 Abstract This report is the result of Master's Degree in Food Engineering at the Agrarian College of Coimbra. The intership took place at the company Frijobel, S.A with headquarters in Espinheiro, Penela, between January the 2nd and the 2nd of July, 2017. The main themes of the project developed are: safety and food quality, emphatic aspects not only for the final consumer but also for the business partners. The management of safety and food quality must be made in a systematic way, by implementing a structured mangement integrated with global activities. There are various normative references for these stuctured management, therefore the requirements of BRC Food, version 7 were implemented, considering there was an interest of Frijobel in obtaining a certificate of that referential. Thus, the main goal of this intership was the implementation, at Frijobel, structured mangement of safety and food quality that can satisfy the requirements of BRC. For that, it was necessary: ✤ The development of a documentary support of the management, having in mind their suitability and posterior control; ✤ The revision of the technical documentation already existed and the criation of the documentary that stil needed to be done; ✤ The criation of the monitoring and control procedures. The goals of this intership were achieved, Frijobel accomplished, on the 20th of June, 2017 the BRC standard certification. Key words: food safety, food quality, structured mangement, BRC Food version 7
- Staining of E-selectin ligands on paraffin-embedded sections of tumor tissuePublication . Carrascal, MA; Talina, C; Borralho, P; Gonçalo Mineiro, A; Henriques, AR; Pen, C; Martins, M; Braga, S; Sackstein, R; Videira, PABACKGROUND: The E-selectin ligands expressed by cancer cells mediate adhesion of circulating cancer cells to endothelial cells, as well as within tissue microenvironments important for tumor progression and metastasis. The identification of E-selectin ligands within cancer tissue could yield new biomarkers for patient stratification and aid in identifying novel therapeutic targets. The determinants of selectin ligands consist of sialylated tetrasaccharides, the sialyl Lewis X and A (sLeX and sLeA), displayed on protein or lipid scaffolds. Standardized procedures for immunohistochemistry make use of the antibodies against sLeX and/or sLeA. However, antibody binding does not define E-selectin binding activity. METHODS: In this study, we developed an immunohistochemical staining technique, using E-selectin-human Ig Fc chimera (E-Ig) to characterize the expression and localization of E-selectin binding sites on paraffin-embedded sections of different cancer tissue. RESULTS: E-Ig successfully stained cancer cells with high specificity. The E-Ig staining show high reactivity scores in colon and lung adenocarcinoma and moderate reactivity in triple negative breast cancer. Compared with reactivity of antibody against sLeX/A, the E-Ig staining presented higher specificity to cancer tissue with better defined borders and less background. CONCLUSIONS: The E-Ig staining technique allows the qualitative and semi-quantitative analysis of E-selectin binding activity on cancer cells. The development of accurate techniques for detection of selectin ligands may contribute to better diagnostic and better understanding of the molecular basis of tumor progression and metastasis.
- Jet properties in PbPb and pp collisions at $ \sqrt{s_{\mathrm{N}\;\mathrm{N}}}=5.02 $ TeVPublication . CMS collaboration (2303 authors); Nayak, Aruna; Bargassa, Pedrame; Beirão Da Cruz E Silva, Cristóvão; Di Francesco, Agostino; Faccioli, Pietro; Galinhas, Bruno; Gallinaro, Michele; Hollar, Jonathan; Leonardo, Nuno; Lloret Iglesias, Lara; Seixas, Joao; Strong, Giles; Toldaiev, Oleksii; Vadruccio, Daniele; Varela, Joao; Vischia, Pietro; David Tinoco Mendes, Andre; Silva, Pedro; Musella, PasqualeModifications of the properties of jets in PbPb collisions, relative to those in pp collisions, are studied at a nucleon-nucleon center-of-mass energy of $ \sqrt{s_{\mathrm{N}\,\mathrm{N}}}=5.02 $ TeV via correlations of charged particles with the jet axis in relative pseudorapidity (Δη), relative azimuth (Δϕ), and relative angular distance from the jet axis $ \varDelta \mathrm{r}=\sqrt{{\left(\varDelta \eta \right)}^2+{\left(\varDelta \phi \right)}^2} $ . This analysis uses data collected with the CMS detector at the LHC, corresponding to integrated luminosities of 404 μb$^{−1}$ and 27.4 pb$^{−1}$ for PbPb and pp collisions, respectively. Charged particle number densities, jet fragmentation functions, and jet shapes are presented as a function of PbPb collision centrality and charged-particle track transverse momentum, providing a differential description of jet modifications due to interactions with the quark-gluon plasma.