Percorrer por autor "Valente, Luísa M. P."
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- Black soldier fly (Hermetia illucens) larvae oil as an alternative to poultry oil in diets for European seabass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata)Publication . Acebes, Daniel; Canada, Paula; Sá, Tiago; Silva, Andreia F.; Magalhães, Sara; Murta, Daniel; Sousa, Ricardo; Valente, Luísa M. P.; Basto, AnaInsects have emerged as an alternative protein source for aquafeeds, and more recently, attention has turned into their lipidic fraction. This study evaluated the potential of black soldier fly larvae oil (BSFLO) to replace poultry oil (PO) in diets for two key Mediterranean aquaculture species: European seabass and gilthead seabream juveniles. A control diet containing 5.2% PO was formulated, along with three experimental diets in which PO was replaced by BSFLO at 33%, 66%, and 100% (1.7%, 3.4%, and 5.2% BSFLO dietary inclusion). Fish were fed to satiation for 8 weeks (gilthead seabream) and 12 weeks (European seabass) at 23.0 ± 0.7 °C and 22.8 ± 0.6 °C, respectively. All diets were well accepted by both species, with no adverse effects on feed intake. Growth performance and feed conversion ratio improved with the full replacement of PO by BSFLO, particularly in seabass. Increasing dietary levels of BSFLO led to greater lauric acid deposition in muscle, enhanced antioxidant capacity, and reduced lipid peroxidation in both species. The complete replacement of PO by BSFLO increased the muscle n-3/n-6 PUFA ratio in both seabass and seabream. Overall, BSFLO proved to be a promising lipid source in aquafeeds for seabass and seabream, with the total PO replacement by BSFLO yielding clear performance gains in seabass and contributing to a more favourable and stable muscle lipid profile (characterized by higher n-3/n-6 ratios, improved antioxidant status, and lower lipid peroxidation) which may support product quality in both species.
- Physiological response of European sea bass (Dicentrarchus labrax) juveniles to an acute stress challenge : the Impact of partial and total dietary fishmeal replacement by an insect mealPublication . Basto, Ana; Peixoto, Diogo; Machado, Marina; Costas, Benjamin; Murta, Daniel; Valente, Luísa M. P.This study aimed to explore the effect of FM substitution by defatted Tenebrio molitor larvae meal (dTM) on the response of European seabass to an acute stress challenge. An FM-based diet was used as a control and two other isoproteic/isoenergetic diets were formulated to replace 50 and 100% of FM by dTM. Each diet was tested in quadruplicate groups of 15 fish (69 ± 5 g) fed until visual satiety for 16 weeks. After the feeding trial, fish were subjected to 1 min air exposure followed by 1 h of recovery before sampling. The haematological profile, plasma metabolites, and humoral immunity biomarkers, as well as hepatic oxidative stress and antioxidant capacity, were analysed. A clear response to acute stress was observed by a significant increase in haemoglobin, haematocrit, red blood cells, and almost all evaluated plasma metabolites and humoral parameters, regardless of dietary treatment. The obtained results demonstrated that partial substitution of FM by IM did not affect the stress response of seabass. However, total FM replacement increased the hepatic activity of total peroxidase and superoxide dismutase in fish fed TM100.