Percorrer por autor "Silvestre, Tiago"
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- Intra and inter-rater repeatability of brachial artery ultrasound estimates of flow-mediated slowing and flow-mediated dilationPublication . Marôco, João Luís; Silvestre, Tiago; Arrais, Inês; Pinto, Marco; Santa-Clara, Helena; Fernhall, Bo; Melo, XavierFlow-mediated slowing (FMS) is a non-invasive measure of endothelial function measured through reactive hyperemia-induced changes in pulse wave velocity (PWV). FMS is suggested to mitigate known pitfalls of flow-mediated dilation (FMD) including suboptimal repeatability and high-operator dependency. However, the few single-rater studies that examined FMS repeatability have shown controversial results and used only regional measurements of PWV, which might not reflect local brachial artery stiffness responses to reactive hyperemia. We assessed the inter- and intra-rater repeatability of ultrasound-based changes in local PWV (FMS) and diameter (FMD). Twenty-four healthy male participants aged 23–75 yr, were examined on two separate days. Reactive hyperemia-induced changes in PWV were calculated using a tailored R-script. The inter- and intra-rater repeatability were tested with the intraclass correlation coefficient (ICC), coefficient of variation (CV), and the Bland-Altman plot estimates. The inter-rater repeatability of FMS (bias: -0.08%; ICC: 0.85; 95% CI: 0.65 to 0.93; CV: 11%) and FMD (bias: -0.02%; ICC: 0.98; 95% CI: 0.97 to 0.99; CV: 7%) showed overall good repeatability over different days. The intra-rater repeatability of FMD (1st rater: bias: 0.27%; ICC: 0.90; 95% CI: 0.78 to 0.96; CV: 14%; 2nd rater: bias: 0.60%; ICC: 0.85; 95% CI: 0.64 to 0.94; CV: 18%) was better than FMS (1st rater: bias: -1.03%; ICC: 0.76; 95% CI: 0.44 to 0.91; CV: 21%; 2nd rater: bias:-0.49%; ICC: 0.70; 95% CI: 0.34 to 0.80; CV: 23%) but not different between raters. Ultrasound-based local measurements of PWV deceleration reactive hyperemia were repeatable among the raters.
- Post-acute exercise cardiovagal modulation in older male adults with and without type 2 diabetesPublication . Marôco, João Luís; Arrais, Inês; Silvestre, Tiago; Pinto, Marco; Laranjo, Sérgio; Magalhães, João; Santa-Clara, Helena; Fernhall, Bo; Melo, XavierPurpose: We examined heart rate variability (HRV) and baroreflex sensitivity (BRS) disease- and age-related response at 10-and 60-min after an acute high-intensity interval (HIIE) and moderate continuous exercise (MICE) in older adults with and without type 2 diabetes mellitus (T2DM) and healthy young adults. Methods: Twelve older male adults with (57–84 years) and without T2DM (57–76 years) and 12 healthy young male adults (20–40 years) completed an isocaloric acute bout of HIIE, MICE, and a non-exercise condition in a randomized order. Time and Wavelets-derived frequency domain indices of HRV and BRS were obtained in a supine position and offline over 2-min time-bins using Matlab. Results: HIIE but not MICE reduced natural logarithm root mean square of successive differences (Ln-RMSSD) (d = − 0.85; 95% CI − 1.15 to − 0.55 ms, p < 0.001), Ln-high-frequency power (d = − 1.60; 95% CI − 2.24 to − 0.97 ms2; p < 0.001), and BRS (d = − 6.32; 95% CI − 9.35 to − 3.29 ms/mmHg, p < 0.001) in adults without T2DM (averaged over young and older adults without T2DM), returning to baseline 60 min into recovery. These indices remained unchanged in older adults with T2DM after HIIE and MICE. Older adults with T2DM had lower resting Ln-RMSSD and BRS than aged-matched controls (Ln-RMSSD, d = − 0.71, 95% CI − 1.16 to − 0.262 ms, p = 0.001; BRS d = − 3.83 ms/mmHg), 95% CI − 6.90 to − 0.76, p = 0.01). Conclusions: Cardiovagal modulation following acute aerobic exercise is intensity-dependent only in adults without T2DM, and appears age-independent. These findings provide evidence of cardiac autonomic impairments in older adults with T2DM at rest and following aerobic exercise.
- Vascular function after acute aerobic exercise in adults with and without type 2 diabetes mellitusPublication . Marôco, João Luís; Arrais, Inês; Silvestre, Tiago; Pinto, Marco; Laranjo, Sérgio; Magalhães, João; Santa-Clara, Helena; Fernhall, Bo; Melo, XavierIt is unknown whether type 2 diabetes mellitus (T2DM) influences the vascular function response to aerobic exercise. We examined brachial artery flow-mediated dilation (FMD) and flow-mediated slowing (FMS) of pulse wave velocity (PWV), 10-and 60-min after a high-intensity interval exercise (HIIE) and moderate-intensity continuous exercise (MICE) in adults with and without T2DM. Twelve older male adults with T2DM (57–84 years), and twenty-four healthy young and older adults (12 per group, aged 20–40 years and 57–76 years, respectively), completed an acute bout of HIIE, MICE, and a non-exercise condition. FMD was evaluated by the same researcher following standardized guidelines. FMS was calculated from the manufacturer’s PWV beta formulas. Central arterial stiffness was estimated via carotid-femoral PWV (cfPWV). %FMD was reduced (d= − 5.94%, 95% CI: − 10.50 to − 1.38%, p = 0.002), whereas %FMS increased (d = 4.55%, 95% CI: 0.62 to 8.48%, p = 0.01), 10-min after HIIE only in adults with T2DM, normalizing 60-min into recovery. Conversely, %FMD was increased (d = 5.33%, 95% CI: 0.76 to 9.89%, p = 0.009) 10-min after MICE only in adults with T2DM. cfPWV remained unchanged following HIIE and MICE in all groups. We report disease-associated vascular function responses to aerobic exercise suggesting both HIIE and MICE uncover transient vascular alterations in older adults with T2DM.