Browsing by Author "Silva, Alexandra Maia e"
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- Análise de polimorfismos no gene humano IGF-IPublication . Sítima, Maria; Dourado-Santos, Tiago; Bernardes, Catarina; Cavaleiro, Inês; Silva, Alexandra Maia e
- Natalizumab treatment modulates Peroxisome Proliferator-Activated Receptors expression in women with multiple sclerosisPublication . Ferret-Sena, Véronique; Silva, Alexandra Maia e; Sena, Armando; Cavaleiro, Inês; Vale, José; Derudas, Bruno; Chinetti-Gbaguidi, Giulia; Staels, BartPeroxisome Proliferator-Activated Receptors (PPAR) are transcription factors suggested to be involved in inflammatory lesions of autoimmune encephalomyelitis and multiple sclerosis (MS). Our objective was to assess whether Natalizumab (NTZ) therapy is associated with alterations of PPAR expression in MS patients. We analyzed gene expression of PPAR in peripheral blood mononuclear cells (PBMC) as well as blood inflammatory markers in women with MS previously medicated with first-line immunomodulators (baseline) and after NTZ therapy. No differences in PPAR𝛼, PPAR𝛽/𝛿, PPAR𝛾, and CD36 mRNA expression were found in PBMC between patients under baseline and healthy controls. At three months, NTZ increased PPAR𝛽/𝛿 mRNA (𝑝 = 0.009) in comparison to baseline, while mRNA expression of PPAR𝛾 and CD36 (a well-known PPAR target gene) was lower in comparison to healthy controls (𝑝 = 0.026 and 𝑝 = 0.028, resp.). Although these trends of alterations remain after six months of therapy, the results were not statistically significant. Osteopontin levels were elevated in patients (𝑝 = 0.002) and did not change during the follow-up period of NTZ
- Rs266882 polymorphism analysis in KLK3 gene by RFLPPCR in cases of prostate cancerPublication . Franco, Joana; Cavaleiro, Inês; Bernardes, Catarina; Ribeiro, Ana Clara; Silva, Alexandra Maia e"The prostate cancer (PC) is one of the most frequent neoplasia in men even though not the deadliest. Every year more than 1,1 million men are diagnose with PC worldwide. The principal risks factors are: age, diet, familiar predisposition, race and hormones. Gleason classification is use to graduate PC based upon the histologic pattern of tumoral growth. Additionally to this classification is use TNM that allow to describe the cancer severity for each individual. Prostate-specific antigen (PSA) has been used as biomarker of PC, however it isn´t a specific marker for cancer detection. This protein is coded by KLK3 gene (located at 19q13.41) and it is regulated by androgen receptors through ligation to consensus sequences in promoter region of KLK3 – Androgen Response Elements (ARE). In ARE-I, located at -158 position, there is a single polymorphism nucleotide (SNP) named rs266882, which consists of substitution of guanine (G) for adenine (A)."
- Vif- H3.3 interaction, a new Vif function?Publication . Silva, Alexandra Maia e; Cavaleiro, Inês; Ribeiro, Ana Clara; Barahona, Isabel