Percorrer por autor "Pina-Martins, Francisco"
A mostrar 1 - 2 de 2
Resultados por página
Opções de ordenação
- Building a Portuguese coalition for biodiversity genomicsPublication . Marques, João P.; Alves, Paulo C.; Amorim, Isabel R.; Lopes, Ricardo J.; Moura, Monica; Myers, Eugene; Sim-sim, Manuela; Sousa-Santos, Carla; Alves, M. Judite; Borges, Paulo A. V.; Brown, Thomas; Carneiro, Miguel; Carrapato, Carlos; Ceríaco, Luís M. P.; Ciofi, Cláudio; Silva, Luís P. da; Diedericks, Genevieve; Diroma, Maria Angela; Farelo, Liliana; Formenti, Giulio; Gil, Fátima; Grilo, Miguel; Iannucci, Alessio; Leitão, Henrique G.; Máguas, Cristina; Mc Cartney, Ann M.; Mendes, Sofia L.; Moreno, João M.; Morselli, Marco; Mouton, Alice; Natali, Chiara; Pereira, Fernando; Rego, Rúben M. C.; Resendes, Roberto; Roxo, Guilherme; Svardal, Hannes; Trindade, Helena; Vicente, Sara; Winkler, Sylke; Alvarenga, Marcela; Amaral, Andreia J.; Antunes, Agostinho; Campos, Paula F.; Canário, Adelino V. M.; Castilho, Rita; Castro, L. Filipe C.; Crottini, Angelica; Cunha, Mónica V.; Themudo, Gonçalo Espregueira; Esteves, Pedro J.; Faria, Rui; Fernandes, Carlos Rodríguez; Ledoux, Jean-Baptiste; Louro, Bruno; Magalhaes, Sara; Paulo, Octávio S.; Pearson, Gareth; Pimenta, João; Pina-Martins, Francisco; Santos, Teresa L.; Serrão, Ester; Melo-Ferreira, José; Sousa, Vítor C.The diverse physiography of the Portuguese land and marine territory, spanning from continental Europe to the Atlantic archipelagos, has made it an important repository of biodiversity throughout the Pleistocene glacial cycles, leading to a remarkable diversity of species and ecosystems. This rich biodiversity is under threat from anthropogenic drivers, such as climate change, invasive species, land use changes, overexploitation, or pathogen (re)emergence. The inventory, characterisation, and study of biodiversity at inter- and intra-specific levels using genomics is crucial to promote its preservation and recovery by informing biodiversity conservation policies, management measures, and research. The participation of researchers from Portuguese institutions in the European Reference Genome Atlas (ERGA) initiative and its pilot effort to generate reference genomes for European biodiversity has reinforced the establishment of Biogenome Portugal. This nascent institutional network will connect the national community of researchers in genomics. Here, we describe the Portuguese contribution to ERGA’s pilot effort, which will generate high-quality reference genomes of six species from Portugal that are endemic, iconic, and/or endangered and include plants, insects, and vertebrates (fish, birds, and mammals) from mainland Portugal or the Azores islands. In addition, we outline the objectives of Biogenome Portugal, which aims to (i) promote scientific collaboration, (ii) contribute to advanced training, (iii) stimulate the participation of institutions and researchers based in Portugal in international biodiversity genomics initiatives, and (iv) contribute to the transfer of knowledge to stakeholders and engaging the public to preserve biodiversity. This initiative will strengthen biodiversity genomics research in Portugal and fuel the genomic inventory of Portuguese eukaryotic species. Such efforts will be critical to the conservation of the country’s rich biodiversity and will contribute to ERGA’s goal of generating reference genomes for European species.
- Who packed the drugs? Improved DNA recovery and its impact on findings given activity-level propositionsPublication . Faria, Sara; Gill, Peter; Ribeiro, Ana Clara; Pina-Martins, Francisco; Fonneløp, Ane ElidaThe interpretation of forensic DNA findings given activity-level propositions depends on the processes governing DNA transfer, persistence, and recovery, as well as on the laboratory methods used to recover DNA. This study investigated the effects of transfer type, substrate characteristics, and DNA extraction strategy on DNA yield, STR profiling performance, and resulting likelihood ratios given activity-level propositions. Direct and indirect transfer experiments were performed using personal bags (backpacks and purses) and zip-lock bags. Three analytical approaches were compared: Chelex extraction, a co-extraction protocol combining the QIAamp DNA Mini Kit and mirVana™ miRNA Isolation Kit, and Direct PCR. Consistent with established transfer principles, direct transfer produced substantially higher DNA quantities than indirect transfer across all methods. The proportion of DNA transferred from bags to zip-lock bags was slightly influenced by bag type and inner surface characteristics. Extraction approach noticeably affected DNA yield and profiling success. The co-extraction protocol generated higher DNA quantities and more robust STR profiles compared with Chelex and Direct PCR. Direct PCR provided advantages in minimizing DNA loss but showed limitations related to inhibition and overall profile quality compared with the co-extraction protocol. Differences in extraction efficiency altered the distributions of DNA quantities observed under direct and indirect transfer scenarios, thereby influencing likelihood ratios given activity-level propositions. These findings are consistent with inter-laboratory studies demonstrating that methodological variation can influence the distributions underlying evaluations of DNA findings given activities. Overall, the results demonstrate that laboratory methodology, particularly the DNA extraction approach, plays a critical role not only in DNA recovery and profiling performance but also in the evidential weight assigned when evaluating DNA findings given activity-level propositions.