Browsing by Author "Morais, Paulo"
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- Genetic portrait of Lisboa immigrant population from Cabo Verde with mitochondrial DNA analysisPublication . Morais, Paulo; Amorim, António; Silva, Cláudia Vieira da; Ribeiro, Teresa; Santos, Jorge Costa; Costa, Heloísa Afonso"The aims of this study were (i) to enrich mtDNA global database, (ii) obtainment of the mtDNA variability of the Cabo Verde population living in Lisboa to complement previous studies by our group using STR genetic markers (Amorim et al. 2012; Afonso Costa et al. 2014), (iii) assign haplotypes to designated haplogroups, (iv) infer whether there are genetic proximity between the studied population and previous studies according to the mtDNA profile of the Cabo Verde population, and (v) compare the studied population with other African populations, with the aim to bring more light to our understanding on the subject of the impact of migrations involving Cabo Verde archipelago’s origin."
- Insertion/Delection Polymorphism and forensic aplications: A preliminary studyPublication . Vieira Da Silva, Cláudia; Matos, Sara; Amorim, António; Afonso Costa, Heloísa; Morais, Paulo; Santos, Rodolfo; Espinheira, Rosa; Santos, J. CostaThe human genetic identification is usually based on the study of STR markers, robust and reliable for samples containing relatively small quantities of DNA. Recent advances in forensic genetics have focused on the development of genotyping assays using shorter amplicons, in order to improve the successful amplification of degraded samples. Single Nucleotide Polymorphisms (SNP) and Insertion/Deletion polymorphisms (INDEL), length polymorphisms created by insertions or deletions of one or more nucleotides in the genome, have considerable potential in this kind of forensic samples, usually present in identification casework, since they can combine desirable characteristics of both, STR and SNP. In this study, a set of 30 biallelic Deletion/Insertion polymorphisms (DIP or INDEL) distributed over 19 autosomes plus Amelogenin in a single multiplex PCR reaction was applied to 100 healthy and unrelated caucasian individuals. Statistical analysis revealed that the 30 biallelic markers can provide satisfactory levels of informativeness for forensic demands.