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Browsing by Author "Clemente, S."

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  • Envelope C2-V3-C3-specific antibodies from HIV-1 infected patients from Angola correlate with neutralization activity in plasma
    Publication . Calado, R.; Duarte, J.; Diniz, A. R.; Borrego, P.; Marcelino, J. M.; Wilton, J.; Bartólo, I.; Clemente, S.; Taveira, N.
    "Development of immunogens that induce broadly neutralizing antibodies (bNAbs) is a major goal in HIV-1 vaccine field. Recently, we found that bNAbs can be elicited in Balb/c mice against HIV-2 by using a prime-boost vaccination strategy combining recombinant Vaccinia virus expressing a truncated form of the SU glycoprotein and a polypeptide comprising the C2, V3 and C3 envelope regions. We want to test the hypothesis that a similar vaccination strategy can also be effective for HIV-1. We also want to test the hypothesis that envelope glycoproteins derived from ancestral HIV-1 isolates from Angola may induce a broader neutralizing antibody response compared to envelope glycoproteins derived from contemporaneous isolates."
    2015-03-22Conference object Open access Show more
  • Evaluation of an in-house molecular HIV-1 test to assess mother-to-child HIV-1 transmission in Angola (the APEHC cohort)
    Publication . Martin, F.; Palladino, C.; Mateus, R.; Clemente, S.; Gomes, P.; Taveira, N.
    "Mother-to-child-transmission (MTCT) rate has decreased sharply in recent years in most of the sub-Saharan Africa, however 220,000 children acquired HIV-1 in 2014. PCR detection of proviral DNA is the most sensitive method for early infant diagnosis (EID) of HIV-1 infection. Commercial kits are available but they have poor sensitivity with divergent non-B subtypes and high costs (≈30€ per test) which limit their use in resource-limited settings. The HIV-1 epidemic in Angola is driven by highly divergent strains of all group M subtypes, except B, as well as multiple recombinant forms (CRFs and URFs) making EID a challenge in this setting. The aim of this study was to develop and validate a qualitative, inexpensive and sensitive “inhouse” HIV-1 EID assay on heel prick dried blood spots (DBS) from infants of the Hospital da Divina Providência (HDP) in Luanda, Angola and determine the current HIV-1 MTCT rate in the Angolan PErinatal HIV Cohort (APEHC)."
    2015-09-27Conference object Open access Show more