Logo do repositório

Percorrer por autor "Bibi, Sagida"

A mostrar 1 - 2 de 2
  • Antiviral responses induced by Tdap-IPV vaccination are associated with persistent humoral immunity to Bordetella pertussis
    Publication . Gillard, Joshua; Suffiotti, Madeleine; Brazda, Peter; Venkatasubramanian, Prashanna B.; Versteegen, Pauline; Jonge, Marien I. de; Kelly, Dominic; Bibi, Sagida; Pinto, Marta Valente; Simonetti, Elles; Babiceanu, Mihaela; Kettring, Andrew; Teodosio, Cristina; Groot, Ronald de; Berbers, Guy; Stunnenberg, Hendrik G.; Schanen, Brian; Fenwick, Craig; Huynen, Martijn A.; Diavatopoulos, Dimitri A.
    Many countries continue to experience pertussis epidemics despite widespread vaccination. Waning protection after booster vaccination has highlighted the need for a better understanding of the immunological factors that promote durable protection. Here we apply systems vaccinology to investigate antibody responses in adolescents in the Netherlands (N = 14; NL) and the United Kingdom (N = 12; UK) receiving a tetanus-diphtheria-acellular pertussis-inactivated poliovirus (Tdap-IPV) vaccine. We report that early antiviral and interferon gene expression signatures in blood correlate to persistence of pertussis-specific antibody responses. Single-cell analyses of the innate response identified monocytes and myeloid dendritic cells (MoDC) as principal responders that upregulate antiviral gene expression and type-I interferon cytokine production. With public data, we show that Tdap vaccination stimulates significantly lower antiviral/type-I interferon responses than Tdap-IPV, suggesting that IPV may promote antiviral gene expression. Subsequent in vitro stimulation experiments demonstrate TLR-dependent, IPV-specific activation of the pro-inflammatory p38 MAP kinase pathway in MoDCs. Together, our data provide insights into the molecular host response to pertussis booster vaccination and demonstrate that IPV enhances innate immune activity associated with persistent, pertussis-specific antibody responses.
    2024-03Contributo em revista Acesso aberto Ver mais
  • A novel whole blood assay to quantify the release of T cell associated cytokines in response to Bordetella pertussis antigens
    Publication . Pinto, Marta Valente; Barkoff, Alex-Mikael; Bibi, Sagida; Knuutila, Aapo; Teräsjärvi, Johanna; Clutterbuck, Elizabeth; Gimenez-Fourage, Sophie; Pagnon, Anke; Gaans-van den Brink, Jacqueline A.M. van; Corbiere, Veronique; Montfort, Aymeric De; Saso, Anja; Jobe, Haddijatou; Roetynck, Sophie; Kampmann, Beate; Simonetti, Elles; Diavatopoulos, Dimitri; Lambert, Eleonora E.; Mertsola, Jussi; Blanc, Pascal; Els, Cécile A.C.M. van; Kelly, Dominic; He, Qiushui; The PERISCOPE Consortium
    Background: Bordetella pertussis continues to cause whooping cough globally even in countries with high immunisation coverage. Booster vaccinations with acellular pertussis vaccines are thus used in children, adolescents, and adults. T cell immunity is crucial for orchestrating the immune response after vaccination. However, T cell assays can be expensive and difficult to implement in large clinical trials. In this study, a whole blood (WB) stimulation assay was developed to identify secreted T cell associated cytokines in different age groups after acellular pertussis booster vaccination. Material and methods: Longitudinal WB samples were collected from a small set of subjects (n = 38) aged 7–70 years participating in a larger ongoing clinical trial. For assay development, samples were diluted and incubated with purified inactivated pertussis toxin (PT), filamentous haemagglutinin (FHA), inactivated B. pertussis lysate, and complete medium (M) as stimulating conditions, with anti-CD28 and anti-CD49d as co-stimulants. Different timepoints around the vaccination (D0, D7, D14, D28), WB dilution factor (1:2, 1:4) and incubation time (24 h, 48 h, 72 h) were compared. Responses to 15 cytokines were tested with Luminex/multiplex immunoassay. Results: The optimized assay consisted of WB incubation with M, PT, and FHA (including the two co-stimulants). After 48 h incubation, supernatants were collected for measurement of seven selected T cell associated cytokines (IL-2, IL-5, IL-10, IL-13, IL-17 A, IL-17F, and IFN-y) from samples before and 28 days after vaccination. PT stimulation showed a trend for upregulation of IL-2, IL-13, and IL-17 A/F for adult subjects, whereas the responses of all cytokines were downregulated for the paediatric subjects. Furthermore, PT and FHA-stimulated WB showed diverse cytokine producing profiles. Conclusions: The developed WB-based cytokine assay was shown to be less costly, easy to perform, and functional in differently aged individuals. Further, it requires only a small amount of fresh blood, which is beneficial especially for studies including infants. Our results support the use of this assay for other immunological studies in the future.
    2024-11Contributo em revista Acesso aberto Ver mais