Browsing by Author "Bandarra, Susana"
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- Are there infectivity differences between HIV-2 and HIV-1 related to APOBEC3?Publication . Bandarra, Susana; Ribeiro, Ana Clara; Gonçalves, João; Barahona, Isabel
- Characterization of CYP2C19*17 polymorphism in a Portuguese population sample relevant for proton pump inhibitor therapy : a pilot studyPublication . Ferraz, Adriana M. L.; Bandarra, Susana; Mascarenhas, Paulo; Barahona, Isabel; Martins, Rui; Ribeiro, Ana ClaraThe interindividual variability of Proton Pump Inhibitor (PPI) therapy results from the phenotype variability associated with the cytochrome P450 2C19 (CYP2C19) gene, namely the CYP2C19*17 allele. Our aim was to characterize patients’ genetic variability undergoing PPI therapy. A sample of 33 oral mucosa cells from Portuguese pharmacy patients was collected, followed by genotyping. The allelic frequencies of CYP2C19*1 (-806C) and CYP2C19*17 (-806T) were 71.2% and 28.8%, respectively. The genotypic frequencies for CYP2C19*1/*1 and CYP2C19*1/*17 were 42.4% and 57.6%, respectively, and 19 of these patients may have a Rapid Metabolizer (RM) phenotype pharmaceutical opinion letter, based on genetic evidence.
- Comparison of in-office and at-home tooth-whitening products cytotoxicityPublication . Pitz, Kristel; Bandarra, Susana; Mascarenhas, Paulo; Ribeiro, Ana Clara; Azul, Ana; Salema-Oom, Madalena; Barahona, Isabel
- Cytotoxicity of temperature-responsive cationic diblock copolymers in human cancer and non-cancer cells linesPublication . Bandarra, Susana; Mascarenhas, Paulo; Barahona, Isabel; Nyströmb, Bo; Calejo, Maria Teresa
- Detection of the SARS-CoV-2 UK variant in PortugalPublication . Bandarra, Susana; Monteiro, Lurdes; Brum, LauraAt the end of 2020, a new highly transmissible variant of SARS-CoV-2 was discovered in the United Kingdom (UK). This work aims to identify potential cases of the UK variant in Portugal using routine diagnostic samples. A total of 26 out of 43 positive samples that were identified by RT-PCR as suspects were confirmed through sequencing to be the SARS-CoV-2 UK variant. The first case of the UK variant identified by us was in samples collected on 21 December 2020 at Lisbon airport in travelers from Manchester and London.
- Effect of Vif in doxorubicin treated breast cancer cellsPublication . Bandarra, Susana; Cipriano, Pedro; Gonçalves, João; Ribeiro, Ana Clara; Barahona, Isabel
- Evaluation of the cytotoxic potential of adhesives, with two on the market: Scotchbond Universal and Optibond Solo Plus, and an adhesive in the experimental phase: T1Publication . Santos, Sofia; Mascarenhas, Paulo; Bandarra, Susana; Ribeiro, Ana Clara; Maurício, Paulo; Barahona, IsabelIn vitro studies evaluating the cytotoxic potential of substances released from dental adhesives are lacking. The purpose of this study was to compare the cytotoxicity of the extracts of dental adhesives Scotchbond Universal and Optibond Solo Plus, and an adhesive in the experimental phase: T1. 3T3 mouse fibroblast cells and MG-63 osteoblast-like cells from human osteosarcoma were exposed for 24 h to serial extract dilutions. Cytotoxicity was determined using an MTT assay. For both cell lines, the cytotoxicity order obtained, of the unfiltered adhesive extracts, was T1 (less cytotoxic) < Optibond Solo Plus < Scotchbond Universal (most cytotoxic).
- HIV Vif protein in docetaxel treatment of breast cancer cellsPublication . Cipriano, Pedro; Bandarra, Susana; Gonçalves, João; Ribeiro, Ana Clara; Barahona, Isabel
- Hyperglycemia phenotype as indicated by salivary glucose biomarkerPublication . Mascarenhas, Paulo; Bandarra, Susana; Ribeiro, Ana Clara; Barahona, Isabel
- Toxicological impact of JWH-018 and its phase I metabolite N-(3-hydroxypentyl) on human cell linesPublication . Couceiro, Joana; Bandarra, Susana; Sultan, Haider; Bell, Suzanne; Constantino, Susana; Quintas, Alexandre"The emergence and abuse of synthetic cannabinoids has been increasing as an alternative to cannabis, mainly among youth. As their appearance on the drug market has been recent, the pharmacological and toxicological profiles of these psychoactive substances are poorly understood. Current studies suggest that they have stronger effects compared to their natural alternatives and their metabolites retain affinity towards CB1 receptors in CNS. Since studies on its toxicological properties are scarce, the effects of the drug in human derived cell lines were investigated. The present study was designed to explore the toxicological impact of parent drug versus phase I metabolites of synthetic cannabinoids on human cells with and without CB1 receptor. The human cell line of neuroblastoma SH-SY5Y and human kidney cell line HEK-293T were exposed to JWH-018 and to its N-(3-hydroxypentyl) metabolite. Cell toxicity was evaluated using the MTT and LDH assay. Additionally, a dual staining methodology with fluorescent Annexin V-FITC and propidium iodide was performed to address the question of whether JWH-018 N-(3-hydroxypentyl) metabolite is inducing cell death through apoptosis or necrosis, in HEK293T and SH-SY5Y cell lines. The obtained results show that JWH-018 does not cause a statistically significant decrease in cell viability, in contrast to its N-(3-hydroxypentyl) metabolite, which at ≥25μM causes a significant decrease in cell viability. Both cell lines are affected by JWH-018 metabolite. Our results point to higher toxicity of JWH-018 metabolite when compared to its parent drug, suggesting a non-CB1 receptor mediated toxicological mechanism. Comparing the results from Annexin V/PI with MTT and LDH assays of SH-SY5Y and HEK293T in the presence of the synthetic cannabinoid metabolite, emerges the picture that cellular viability decreases and associated death is occurring through necrosis."