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Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water

1996-05Journal article Open access
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dc.contributor.authorFerreira, A.C.
dc.contributor.authorMorais, Paula V
dc.contributor.authorAlcobia Gomes, Célia Margarida
dc.contributor.authorDa Costa, Milton
dc.date.accessioned2020-10-07T12:05:31Z
dc.date.available2020-10-07T12:05:31Z
dc.date.issued1996-05
dc.description.abstractThe microflora of a natural mineral water was studied immediately after bottling (T0) and after 7 d storage (T7) during 6 months, and isolates were clustered by SDS-PAGE of whole-cell protein profiles. Isolates from each cluster were further characterized by API 20NE, fatty acid composition and quinone profiles. The numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. Except for five minor clusters, all clusters were composed of strains isolated over several months. The numerical analysis of the electrophoregrams of bacteria isolated immediately after bottling formed 15 clusters while after 7 d storage only four of these populations could be isolated, indicating that populations present in the mineral water were stable and that changes occurring after bottling probably resulted from a selection process. Only one unclustered strain was identified simultaneously by all the systems, as Sphingomonas paucimobilis. The monitoring of the aquifer and the bottling system, and the construction of a large database with bacteria of the autochthonous flora allows the detection of alterations in the aquifer by changes in the microflora.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1111/j.1365-2672.1996.tb03246.xpt_PT
dc.identifier.issn0021-8847
dc.identifier.urihttp://hdl.handle.net/10400.26/33490
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley Blackwellpt_PT
dc.relation.publisherversionhttps://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.1996.tb03246.xpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/pt_PT
dc.subjectBacteriapt_PT
dc.subjectBacterial proteinspt_PT
dc.subjectFatty acidspt_PT
dc.subjectMineral waterspt_PT
dc.subjectReproducibility of resultspt_PT
dc.subjectTime factorspt_PT
dc.subjectElectrophoresis, polyacrylamide gelpt_PT
dc.subjectNumerical analysis, computer-assistedpt_PT
dc.subjectQuinonespt_PT
dc.subjectBactériaspt_PT
dc.subjectProteínas de bactériaspt_PT
dc.subjectÁcidos gordospt_PT
dc.subjectÁguas mineraispt_PT
dc.subjectReprodutibilidade dos resultadospt_PT
dc.subjectEletroforese em gel de poliacrilamidapt_PT
dc.subjectAnálise numérica assistida por computadorpt_PT
dc.subjectQuinonaspt_PT
dc.subjectFatores de tempopt_PT
dc.titleComputer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral waterpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage486pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage479pt_PT
oaire.citation.titleJournal of Applied Bacteriologypt_PT
oaire.citation.volume80pt_PT
person.familyNameMorais
person.familyNameAlcobia Gomes
person.familyNameda Costa
person.givenNamePaula Vasconcelos
person.givenNameCélia Margarida
person.givenNameMilton
person.identifier185035
person.identifier.ciencia-idF313-B890-9B46
person.identifier.ciencia-id061F-EB4B-4A76
person.identifier.orcid0000-0002-1939-6389
person.identifier.orcid0000-0001-6329-8527
person.identifier.orcid0000-0003-0498-1005
person.identifier.ridC-9756-2014
person.identifier.scopus-author-id7005946097
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7b307fe5-d5e8-4175-b3e5-aa19c8bf7c12
relation.isAuthorOfPublication7f538554-a538-4c9d-8971-b66d484aafce
relation.isAuthorOfPublication78593aa4-a3e6-4497-b808-4511083196eb
relation.isAuthorOfPublication.latestForDiscovery7f538554-a538-4c9d-8971-b66d484aafce

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