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Cold atmospheric plasma, a novel approach against bladder cancer, with higher sensitivity for the high-grade cell line

2021-01-09Journal article Open access
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dc.contributor.authorTavares-da-Silva, Edgar
dc.contributor.authorPereira, Eurico
dc.contributor.authorPires, Ana S.
dc.contributor.authorNeves, Ana R.
dc.contributor.authorBraz-Guilherme, Catarina
dc.contributor.authorMarques, Inês A.
dc.contributor.authorAbrantes, Ana M
dc.contributor.authorGonçalves, Ana C.
dc.contributor.authorCaramelo, Francisco
dc.contributor.authorSilva-Teixeira, Rafael
dc.contributor.authorMendes, Fernando
dc.contributor.authorFigueiredo, Arnaldo
dc.contributor.authorBotelho, Maria Filomena
dc.date.accessioned2021-02-22T23:16:12Z
dc.date.available2021-02-22T23:16:12Z
dc.date.issued2021-01-09
dc.description.abstractAntitumor therapies based on Cold Atmospheric Plasma (CAP) are an emerging medical field. In this work, we evaluated CAP effects on bladder cancer. Two bladder cancer cell lines were used, HT-1376 (stage III) and TCCSUP (stage IV). Cell proliferation assays were performed evaluating metabolic activity (MTT assay) and protein content (SRB assay). Cell viability, cell cycle, and mitochondrial membrane potential (Δψm) were assessed using flow cytometry. Reactive oxygen and nitrogen species (RONS) and reduced glutathione (GSH) were evaluated by fluorescence. The assays were carried out with different CAP exposure times. For both cell lines, we obtained a significant reduction in metabolic activity and protein content. There was a decrease in cell viability, as well as a cell cycle arrest in S phase. The Δψm was significantly reduced. There was an increase in superoxide and nitric oxide and a decrease in peroxide contents, while GSH content did not change. These results were dependent on the exposure time, with small differences for both cell lines, but overall, they were more pronounced in the TCCSUP cell line. CAP showed to have a promising antitumor effect on bladder cancer, with higher sensitivity for the high-grade cell line.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/biology10010041pt_PT
dc.identifier.issn2079-7737
dc.identifier.urihttp://hdl.handle.net/10400.26/35612
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationCNC. IBILI
dc.relationUNREAD - RadiUm-223 iN metastatic pRostatE cAncer: searching the keys beyond clinical eviDence
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/pt_PT
dc.subjectCold atmospheric plasmapt_PT
dc.subjectPlasma medicinept_PT
dc.subjectBladder cancerpt_PT
dc.subjectUrinary bladder neoplasmspt_PT
dc.subjectReactive oxygen and nitrogen speciespt_PT
dc.subjectCell deathpt_PT
dc.titleCold atmospheric plasma, a novel approach against bladder cancer, with higher sensitivity for the high-grade cell linept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCNC. IBILI
oaire.awardTitleUNREAD - RadiUm-223 iN metastatic pRostatE cAncer: searching the keys beyond clinical eviDence
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FNEU%2F04539%2F2019/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04539%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage41pt_PT
oaire.citation.titleBiologypt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
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oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGonçalves Pereira
person.familyNameAbrantes
person.familyNameCaramelo
person.familyNameSilva-Teixeira
person.familyNameMendes
person.familyNameCastro Figueiredo
person.familyNameRabaça Roque Botelho
person.givenNameEurico José
person.givenNameAna M
person.givenNameFrancisco
person.givenNameRafael
person.givenNameFernando
person.givenNameArnaldo José
person.givenNameMaria Filomena
person.identifierR-000-10V
person.identifierG-1860-2011
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person.identifier.orcid0000-0002-0015-8604
person.identifier.orcid0000-0002-8151-4024
person.identifier.orcid0000-0002-5205-8939
person.identifier.orcid0000-0002-7157-0081
person.identifier.orcid0000-0001-7202-1650
person.identifier.ridG-6913-2018
person.identifier.scopus-author-id26029747600
person.identifier.scopus-author-id14071115100
person.identifier.scopus-author-id7005751167
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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