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Assessment of DNA and mtDNA Degradation in Sperm Cells Collected by Laser Micro-dissection

2017Journal article Open access
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dc.contributor.authorLima, Gabriela
dc.contributor.authorCorreia de Sá, P
dc.contributor.authorPorto, MJ
dc.contributor.authorCainé, Laura
dc.contributor.authorCosta, S
dc.date.accessioned2023-02-16T11:07:51Z
dc.date.available2023-02-16T11:07:51Z
dc.date.issued2017
dc.description.abstractSexual assault samples are among the most frequently analyzed in a forensic laboratory. These accounts for almost half of all samples processed routinely and a large portion of these cases remain unsolved. These samples often pose problems to traditional analytic methods of identification because they consist most frequently of cell mixtures from at least two contributors: the victim (usually female) and the perpetrator (usually male). In this study we prepared simulated sexual assault samples and conducted amplification and DNA genotyping from sperm cells collected with laser microdissection at different time intervals to assess the DNA degradation at those intervals. Furthermore, we then investigated the possibility of recovering mtDNA from the collected sperm cells, particularly in cases where autosomal DNA was not suitably amplified. With this work we determined that it becomes possible to extend the timeframe for performing an analysis, by researching other sources of DNA, namely mtDNA.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta s, Lima G, Correia-de-Sa P, Porto MJ, Caine L (2017) Assessment of DNA and mtDNA Degradation in Sperm Cells Collected by Laser Micro-dissection. J Forensic Res 8: 393. doi:10.4172/2157-7145.1000393pt_PT
dc.identifier.doi10.4172/2157-7145.1000393pt_PT
dc.identifier.issn2157-7145
dc.identifier.urihttp://hdl.handle.net/10400.26/43868
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectSexual assaultpt_PT
dc.subjectSpermpt_PT
dc.subjectmtDNApt_PT
dc.subjectDegraded Samplept_PT
dc.subjectLaser Microdissectionpt_PT
dc.titleAssessment of DNA and mtDNA Degradation in Sperm Cells Collected by Laser Micro-dissectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleJournal of Forensic Researchpt_PT
oaire.citation.volume8(5)pt_PT
person.familyNameCainé
person.givenNameLaura
person.identifierAAR-9087-2020
person.identifier.ciencia-id021A-33DD-869D
person.identifier.orcid0000-0002-6542-4256
person.identifier.scopus-author-id2311978
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1a1e1ffc-1439-48f2-a4c3-359c66db3ad1
relation.isAuthorOfPublication.latestForDiscovery1a1e1ffc-1439-48f2-a4c3-359c66db3ad1

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