Forensic Genetics as a Tool for Peace and Justice: An Overview on DNA Quantification

Vieira Da Silva, Cláudia; Afonso Costa, Heloísa; Costa Santos, J.; Espinheira, Rosa

Publication

Forensic Genetics as a Tool for Peace and Justice: An Overview on DNA Quantification

2012-01Journal article

dc.contributor.author	Vieira Da Silva, Cláudia
dc.contributor.author	Afonso Costa, Heloísa
dc.contributor.author	Costa Santos, J.
dc.contributor.author	Espinheira, Rosa
dc.date.accessioned	2022-07-12T16:12:31Z
dc.date.available	2022-07-12T16:12:31Z
dc.date.issued	2012-01
dc.description.abstract	In Forensic Genetics, DNA analysis is performed to obtain a Short Tandem Repeat (STR) profile from an evidence sample, which is then compared with the victim and suspect(s) reference sample STR profile, to determine their contribution to that evidence sample. However, forensic biological samples can be present in low quantities and be exposed to different environmental insults leading to DNA degradation and contamination by inhibitor compounds. Thus, it is desirable for a forensic scientist to have useful information about the forensic sample quantity and quality prior to STR amplification. New methods in Forensic DNA analysis for detecting, preserving, and quantifying DNA, as well as its recovery from different biological materials are continually being developed. Real-Time PCR (RT-PCR) assays for DNA quantification, like the recent Quantifiler® Duo DNA quantification kit (Applied Biosystems) proved to be very useful in forensic samples. Since many samples, mainly those resulting from sexual assault cases are often composed by unbalanced male/female DNA mixtures, the knew RT-PCR quantification assay, developed to quantify relative male/ female DNA ratio contributes not only to total DNA determination but also to ascertain the presence and quantity of enough male DNA in the sample. These results are important to guide the optimal STR analysis selection, such as autosomal STR, Y-STR, or mini-STR, increasing downstream analysis success rates. In this work we present real forensic casework where the DNA amount and quality were important to guide the selection of the appropriate STR amplification kit in order to increase the success of profiling in the first attempt, reducing the number of samples that need to be reprocessed and thereby decreasing the turn around time in a forensic laboratory.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Silva, C. V. da, Costa, H. A., Santos, J. C., & Espinheira, R. (2012). Forensic Genetics as a Tool for Peace and Justice: An Overview on DNA Quantification. Journal of Forensic Research, 04(01)	pt_PT
dc.identifier.doi	10.4172/2157-7145.S11-008	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.26/41315
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	OMICS Publishing Group	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/4.0/	pt_PT
dc.subject	Forensic genetics	pt_PT
dc.subject	DNA quantification	pt_PT
dc.subject	RT-PCR	pt_PT
dc.subject	STR	pt_PT
dc.subject	Quantifiler Duo	pt_PT
dc.title	Forensic Genetics as a Tool for Peace and Justice: An Overview on DNA Quantification	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.issue	01	pt_PT
oaire.citation.title	Journal of Forensic Research	pt_PT
oaire.citation.volume	04	pt_PT
person.familyName	da Silva
person.familyName	Afonso Costa
person.familyName	Santos
person.givenName	Cláudia
person.givenName	Heloísa
person.givenName	Jorge
person.identifier.ciencia-id	D218-0D74-678B
person.identifier.orcid	0000-0003-0373-7457
person.identifier.orcid	0000-0002-7823-7687
person.identifier.orcid	0000-0002-8925-3068
person.identifier.rid	J-5725-2015
person.identifier.scopus-author-id	9839086200
person.identifier.scopus-author-id	24729171700
person.identifier.scopus-author-id	7402389332
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
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