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Chromatographic determination of antidepressants in plasma and saliva: Towards non-invasive therapeutic monitoring

2025-12-15Artigo científico Acesso aberto
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dc.contributor.authorSoares, Sofia
dc.contributor.authorRosendo, Luana
dc.contributor.authorFonseca, Suzana
dc.contributor.authorGonçalves, Nuno
dc.contributor.authorFranco, João
dc.contributor.authorda Costa, António Pissarra
dc.contributor.authorRosado, Tiago
dc.contributor.authorBarroso, Mário
dc.contributor.authorSantos, Vítor Hugo
dc.contributor.authorRei, Cristina
dc.contributor.authorAmantegui, Patricia
dc.contributor.authorChaves, Telma
dc.contributor.authorValente, Rita
dc.contributor.authorDuarte, Fábio
dc.contributor.authorPacheco, Susana
dc.contributor.authorMartins, Marco
dc.contributor.authorDias, Kátia
dc.contributor.authorCosta, Patricia
dc.contributor.authorCosta, Rui
dc.contributor.authorCastro, Sílvia
dc.contributor.authorSousa, Diana
dc.contributor.authorFigueiredo, Diana
dc.contributor.authorSoares, Isabel
dc.contributor.authorMouta, Salomé
dc.contributor.authorJesus, Bianca
dc.contributor.authorPires, Ana
dc.contributor.authorRibeiro, Cândida
dc.contributor.authorLobo, Sónia
dc.contributor.authorCorreia, Leonor
dc.contributor.authorMalés, Sofia
dc.contributor.authorVale, Fátima
dc.contributor.authorMoita, Carina
dc.contributor.authorMoura, Carolina
dc.contributor.authorSousa, Joana
dc.contributor.authorAfonso, Luís Rafael
dc.contributor.authorCosta, Rita Santinho
dc.contributor.authorGallardo, Eugenia
dc.date.accessioned2026-01-29T17:48:01Z
dc.date.available2026-01-29T17:48:01Z
dc.date.issued2025-12-15
dc.description.abstractDrug monitoring of antidepressants in plasma and oral fluid represents a valuable tool in clinical practice, enabling the optimisation of treatment efficacy and the reduction of adverse effects. Given the significant interindividual variability in antidepressant response-driven by factors such as metabolism, drug-drug interactions, and adherence to therapy-drug monitoring facilitates dose adjustment based on measured drug concentrations, ensuring levels remain within the therapeutic window. This study aimed at developing and validating a robust, rapid, and sensitive method for the simultaneous quantification of 21 selected antidepressants and their metabolites in only 100 μL of plasma and oral fluid. Sample preparation was performed using a simple protein precipitation protocol, followed by analysis via liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method was validated in accordance with internationally accepted bioanalytical guidelines, demonstrating linearity over the concentration range of 0.98-1000 ng/mL. Limits of quantification were established at 0.98 ng/mL for all analytes across both matrices. The extraction procedure yielded high recovery rates, and the method showed excellent selectivity, sensitivity, precision, and accuracy, confirming its suitability for routine toxicological applications. The validated method was successfully applied to 142 paired authentic plasma and oral fluid specimens from patients undergoing antidepressant therapy. Antidepressant concentrations were determined in both matrices, and treatment adherence was considered high, being confirmed in 88.7 % of patients. Correlation analysis between plasma and oral fluid concentrations produced promising results for several of the compounds under investigation, reinforcing the potential utility of oral fluid as a non-invasive alternative matrix in drug monitoring.eng
dc.identifier.citationSoares, S., Rosendo, L., Fonseca, S., Gonçalves, N., Franco, J. M., Rosado, T., Barroso, M., Santos, V. H., Rei, C., Amantegui, P., da Costa, A. P., Chaves, T., Valente, R., Duarte, F., Pacheco, S., Martins, M., Dias, K., Costa, P., Costa, R., Castro, S., … Gallardo, E. (2025). Chromatographic determination of antidepressants in plasma and saliva: Towards non-invasive therapeutic monitoring. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1267, 124782.
dc.identifier.doihttps://doi.org/10.1016/j.jchromb.2025.124782
dc.identifier.issn1873-376X
dc.identifier.urihttp://hdl.handle.net/10400.26/61314
dc.language.isoen
dc.peerreviewedyes
dc.publisherElsevier
dc.relationThe potential use of oral fluid to monitor antidepressants in clinical and forensic scenarios
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAntidepressants
dc.subjectCorrelation
dc.subjectDrug monitoring
dc.subjectLC-MS/MS
dc.subjectOral fluid
dc.subjectPlasma
dc.subjectProtein precipitation
dc.titleChromatographic determination of antidepressants in plasma and saliva: Towards non-invasive therapeutic monitoringund
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleThe potential use of oral fluid to monitor antidepressants in clinical and forensic scenarios
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F148753%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.citation.titleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences
oaire.citation.volume1267
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameFonseca
person.familyNameFranco
person.familyNameBarroso
person.givenNameSuzana
person.givenNameJoão
person.givenNameMário
person.identifier.ciencia-idC31D-C5F1-D6BD
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0000-0001-9122-1017
person.identifier.orcid0000-0002-3268-3841
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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