Publication
A fast and reliable method for GHB quantitation in whole blood by GC–MS/MS (TQD) for forensic purposes
| dc.contributor.author | Castro, André Lobo | |
| dc.contributor.author | Tarelho, Sónia | |
| dc.contributor.author | Dias, Mário | |
| dc.contributor.author | Reis, Flávio | |
| dc.contributor.author | Teixeira, Helena M. | |
| dc.date.accessioned | 2023-02-28T16:39:18Z | |
| dc.date.available | 2023-02-28T16:39:18Z | |
| dc.date.issued | 2015-11-28 | |
| dc.description.abstract | Gamma-Hydroxybutyric Acid (GHB) is an endogenous compound with a story of clinical use since the 1960’s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market. A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC-MS/MS (TQD) in whole blood is presented. Using 100 µL of whole blood, obtained results included a LOD and LLOQ of 0.1 mg/L and a recovery of 86% in a working range between 0.1 and 100 mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors’ knowledge, the first GC-MS-MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D6 (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample. | pt_PT |
| dc.description.version | info:eu-repo/semantics/acceptedVersion | pt_PT |
| dc.identifier.citation | Castro AL, Tarelho S, Dias M, Reis F, Teixeira HM, “A fast and reliable method for GHB quantitation in whole blood by GC–MS/MS (TQD) for forensic purposes”, Journal of Pharmaceutical and Biomedical Analysis, 119 (2016) 139–144. | pt_PT |
| dc.identifier.doi | 10.1016/j.jpba.2015.11.038 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.26/43979 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0731708515302612?via%3Dihub | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Forensic Science | pt_PT |
| dc.subject | Gamma-Hydroxybutyric Acid (GHB) | pt_PT |
| dc.subject | GC-MS/MS | pt_PT |
| dc.subject | Analytical validation | pt_PT |
| dc.subject | Endogenous values | pt_PT |
| dc.subject | Forensic toxicology | pt_PT |
| dc.title | A fast and reliable method for GHB quantitation in whole blood by GC–MS/MS (TQD) for forensic purposes | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 144 | pt_PT |
| oaire.citation.startPage | 139 | pt_PT |
| oaire.citation.title | Journal of Pharmaceutical and Biomedical Analysis | pt_PT |
| oaire.citation.volume | 119 | pt_PT |
| person.familyName | Lobo Castro | |
| person.familyName | Tarelho | |
| person.familyName | Dias | |
| person.familyName | Fernandes Reis | |
| person.familyName | Teixeira | |
| person.givenName | André | |
| person.givenName | Sónia | |
| person.givenName | Mário | |
| person.givenName | Flávio Nelson | |
| person.givenName | Helena M. | |
| person.identifier.ciencia-id | 1C1D-1326-C311 | |
| person.identifier.ciencia-id | 4214-85EB-E58C | |
| person.identifier.orcid | 0000-0002-7258-8523 | |
| person.identifier.orcid | 0000-0001-8366-6702 | |
| person.identifier.orcid | 0000-0002-5900-5899 | |
| person.identifier.orcid | 0000-0003-3401-9554 | |
| person.identifier.orcid | 0000-0001-7570-5521 | |
| person.identifier.scopus-author-id | 26221673200 | |
| person.identifier.scopus-author-id | 7003981256 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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