The cytotoxic effect of THJ-018 in human neuroblastoma SH-SY5Y cells

Khosasih, Vivia

http://hdl.handle.net/10400.26/19081

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Authors

Khosasih, Vivia

Advisor(s)

Quintas, Alexandre

Costa, Joana Couceiro da

Abstract(s)

Since its first appearing on 2004, synthetic cannabinoidsm the market of the so-called recreational drug, have regained popularity among adolescents and young adults. Current in vitro studies show that synthetic cannabinoids have stringer binding affinity to cannabinoids 1 (CB1) receptor than the Δ9-tetrahydrocannabinal. Therefore, it suggested that synthetic cannabinoids possess stronger psychotropic effect than cannabis. Albeit it has been increasingly abused as the legal alternative of cannabis, the pharmacological and toxicological profiles of synthetic cannabinoids remais poorly understood. One of the earliest synthetic cannabinoids to be identified was JWH-018. This study was performed to explore the toxicological effect of synthetic cannabinoids THJ-018 as the legal analog to JWH-018 on human cells model. SH-SY5Y cell lineage was used as the human cell model in this study since it has been reported to express cannabinoids 1 receptor (CB1). Seven designated concentrations of synthetic cannabinoids THJ-018, such as 1 μM, 5 μM, 10 μM, 25 μM, 50 μM, 75 μM and 100 μM were exposed for 24 hours to human neuroblastoma SH-SY5Y cell lineage. The cell viability was evaluated by performing the 3-(4,5-dimethylthiazolyl-2)-2,5 diphenyltetrazolium bromide (MTT) assay. The MTT results on SH-SY5Y cells show a statiscally significant increase on cell viability (* p<0.05, ** p<0.01 or *** p<0.001) for the concentration of 10, 25, 50, 75 and 100 μM of THJ-018. This results indicated that the THJ-018 is not toxic to the SH-SY5Y cell line. Furthermore, the cytotoxicity effect of THJ-018 combustion product, which is performed to evaluate the mimicry of the process of smoking as the common route of administration for synthetic cannabinoids, show a decrease of cell viability when SH-SY5Y cell exposed with THJ-018 combustion product.