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Comparação de dois diluidores comerciais em parâmetros de qualidade no sémen equino em diferentes tempos
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Advisor(s)
Abstract(s)
Na indústria equina, cada vez mais produtores recorrem à inseminação artificial e,
por essa razão, à colheita de sémen. Uma boa avaliação reprodutiva é muito importante.
Combinando a observação microscópica dos espermatozóides, a análise com sistemas
computadorizados e a citometria de fluxo pode-se obter um alto nível de previsão da
fertilidade. O objetivo geral do trabalho foi comparar dois diluidores comerciais, o INRA
96 ® e o Equiplus® em sémen de 10 garanhões do Centro de Seleção e Reprodução Animal
de Badajoz, com fertilidade provada, em fresco, às 24 e 48h. A colheita do sémen foi feita
com recurso a vaginas artificiais. Foi realizada a avaliação seminal com determinação do
volume, concentração, motilidade (total e progressiva) e pH. Foram comparados os dados
de motilidade e ph em fresco, às 24 e 48h. Por citometria de fluxo foi avaliado o potencial
de membrana mitocondrial, o número de espermatozóides vivos produtores de espécies
reativas de oxigénio e o estado dos acrossomas às 0h e às 48h. A motilidade total (MT) e a
motilidade progressiva (MP) com o diluidor INRA 96®
sofreram menos alterações às 0h,
24h e 48h. O pH do sémen permaneceu dentro de valores mais próximos dos fisiológicos
com os dois diluidores. O uso do INRA 96® forneceu resultados estatisticamente melhores
no que respeita à viabilidade espermática. Não foram encontradas diferenças significativas
no potencial de membrana mitocondrial mas a integridade do acrossoma esteve significativamente mais comprometida, às 48h, nas diluições com Equiplus® . Parece que o INRA 96® fornece melhores resultados na refrigeração do sémen de garanhão às 0, 24 e 48h, sendo para este último tempo estatisticamente significativo.
In the equine industry, the number of producers using artificial insemination has been increasing, leading to an increase in semen collection. An accurate reproductive assessment is crucial. Combining microscopic observation of spermatozoa, analysis with computerized systems and flow cytometry is possible to obtain a high level of fertility prediction. The main goal of this study was to compare two commercial extenders, INRA 96® and Equiplus® semen from 10 stallions living in Badajoz’s Animal Selection and Reproduction Center, with proven fertility, in fresh, at 24h and at 48h. Semen collection was made using artificial vaginas. Seminal evaluation was performed with determination of volume, concentration, motility (total and progressive) and pH. Motility and pH’s values in fresh, were compared with values 24h and 48h after. The mitochondrial membrane potential, the number of live spermatozoa producing reactive oxygen species and the state of the acrosomes, were evaluated using flow cytometry, at 0h and at 48h. Total motility (MT) and progressive motility (MP) underwent less changes at 0h, 24h and 48h, with INRA 96® extender. Semen’s pH remained within values closer to the physiological ones with both extenders. The use of INRA 96® provided statistically better results regarding sperm viability. No significant differences were found in mitochondrial membrane potential, but the acrosome integrity was significantly more compromised, at 48h, with the dilutions with Equiplus® It seems that INRA 96® provides better results in the stallion semen refrigeration at 0h, 24h and 48h, being statistically significant in this latter time period.
In the equine industry, the number of producers using artificial insemination has been increasing, leading to an increase in semen collection. An accurate reproductive assessment is crucial. Combining microscopic observation of spermatozoa, analysis with computerized systems and flow cytometry is possible to obtain a high level of fertility prediction. The main goal of this study was to compare two commercial extenders, INRA 96® and Equiplus® semen from 10 stallions living in Badajoz’s Animal Selection and Reproduction Center, with proven fertility, in fresh, at 24h and at 48h. Semen collection was made using artificial vaginas. Seminal evaluation was performed with determination of volume, concentration, motility (total and progressive) and pH. Motility and pH’s values in fresh, were compared with values 24h and 48h after. The mitochondrial membrane potential, the number of live spermatozoa producing reactive oxygen species and the state of the acrosomes, were evaluated using flow cytometry, at 0h and at 48h. Total motility (MT) and progressive motility (MP) underwent less changes at 0h, 24h and 48h, with INRA 96® extender. Semen’s pH remained within values closer to the physiological ones with both extenders. The use of INRA 96® provided statistically better results regarding sperm viability. No significant differences were found in mitochondrial membrane potential, but the acrosome integrity was significantly more compromised, at 48h, with the dilutions with Equiplus® It seems that INRA 96® provides better results in the stallion semen refrigeration at 0h, 24h and 48h, being statistically significant in this latter time period.
Description
Keywords
INRA 96® Equiplus® sémen diluidor. semen extenders
Pedagogical Context
Citation
Máximo, M.G.C. (2022). Comparação de dois diluidores comerciais em parâmetros de qualidade no sémen equino em diferentes tempos, Escola Superior Agrária de Elvas, Elvas
Publisher
Escola Superior Agrária de Elvas