Publication
Analysis of organophosphorus pesticides in whole blood by GC-MS-μECD with forensic purposes
| dc.contributor.author | Valente, Nuno I.P. | |
| dc.contributor.author | Tarelho, Sónia | |
| dc.contributor.author | Castro, André Lobo | |
| dc.contributor.author | Silvestre, Armando | |
| dc.contributor.author | Teixeira, Helena M. | |
| dc.date.accessioned | 2023-02-17T16:10:45Z | |
| dc.date.available | 2023-02-17T16:10:45Z | |
| dc.date.issued | 2015-03-28 | |
| dc.description.abstract | In the present work, two multi-residue methods for the determination of ten organophosphorus pesticides (OPs), namely chlorfenvinphos, chlorpyrifos, diazinon, dimethoate, fenthion, malathion, parathion, phosalone, pirimiphos-methyl and quinalphos, in post-mortem whole blood samples are presented. The adopted procedure uses GC-MS for screening and quantitation, and GC-µECD (electron capture detector) for compound confirmation. Three different Solid Phase Extraction (SPE) procedures for OPs with Oasis® hydrophilic lipophilic balanced (HLB) and Sep-Pak® C18 cartridges were tested, and followed by GC-µECD and GC-MS analysis. The Sep-Pak® C18 cartridges extraction procedure was selected since it generated analytical signals 5 times higher than those obtained with the two different Oasis® HLB cartridges extraction procedures. The method has shown to be selective for the isolation of selected OPs as well as to the chosen internal standard (ethion) in postmortem blood samples. Calibration curves between 50 and 5000 ng/mL were prepared using weighted linear regression models (1/x2). It was not possible to establish a working range for fenthion by GC-µECD due to the lower sensitivity of the detector to this compound, whereas for pirimiphos-methyl it was set between 500 and 5000 ng/mL. The limit of quantitation was established at 50 ng/mL for all analytes, except for pirimiphos-methyl by GC-µECD analysis (500 ng/mL). The average extraction efficiency ranged from 72 to 102%. The developed methods were considered robust and fit for the purpose, and had already been adopted in the laboratory routine analysis. | pt_PT |
| dc.description.version | info:eu-repo/semantics/acceptedVersion | pt_PT |
| dc.identifier.citation | Valente NIP, Tarelho S, Castro AL, Silvestre A, Teixeira HM, “Analysis of organophosphorus pesticides in whole blood by GC-MS-mECD with forensic purposes”, Journal of Forensic and Legal Medicine, 33 (2015) 28-34. | pt_PT |
| dc.identifier.doi | 10.1016/j.jflm.2015.03.006 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.26/43905 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation | CICECO-Aveiro Institute of Materials | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | GC-MS-ECD | pt_PT |
| dc.subject | Analytical method validation | pt_PT |
| dc.subject | Organophosphorus pesticides | pt_PT |
| dc.subject | Post-mortem blood samples | pt_PT |
| dc.subject | SPE Sep-Pak C18 cartridges | pt_PT |
| dc.title | Analysis of organophosphorus pesticides in whole blood by GC-MS-μECD with forensic purposes | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | CICECO-Aveiro Institute of Materials | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FCTM%2F50011%2F2013/PT | |
| oaire.citation.endPage | 34 | pt_PT |
| oaire.citation.startPage | 28 | pt_PT |
| oaire.citation.title | Journal of Forensic and Legal Medicine | pt_PT |
| oaire.citation.volume | 33 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| person.familyName | Valente | |
| person.familyName | Tarelho | |
| person.familyName | Lobo Castro | |
| person.familyName | Domingues Silvestre | |
| person.familyName | Teixeira | |
| person.givenName | Nuno I.P. | |
| person.givenName | Sónia | |
| person.givenName | André | |
| person.givenName | Armando Jorge | |
| person.givenName | Helena M. | |
| person.identifier | E-6201-2010 | |
| person.identifier.ciencia-id | DC18-EB22-B357 | |
| person.identifier.ciencia-id | 4214-85EB-E58C | |
| person.identifier.orcid | 0000-0001-7003-1628 | |
| person.identifier.orcid | 0000-0001-8366-6702 | |
| person.identifier.orcid | 0000-0002-7258-8523 | |
| person.identifier.orcid | 0000-0001-5403-8416 | |
| person.identifier.orcid | 0000-0001-7570-5521 | |
| person.identifier.scopus-author-id | 7005219944 | |
| person.identifier.scopus-author-id | 7003981256 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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