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Characterization of Legionella species by numerical analysis of whole-cell protein electrophoresis

1996-01-01Journal article Open access
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dc.contributor.authorVeríssimo, António
dc.contributor.authorMorais, Paula V.
dc.contributor.authorDiogo, Alexandra
dc.contributor.authorAlcobia Gomes, Célia Margarida
dc.contributor.authorCosta, Milton S. da
dc.date.accessioned2020-10-07T09:57:31Z
dc.date.available2020-10-07T09:57:31Z
dc.date.issued1996-01-01
dc.description.abstractThe results of a computer-assisted whole-cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of 291 isolates and 74 reference strains belonging to all known species of the genus Legionella revealed that the majority of the species of this genus can be adequately identified by this method. The type strain of Legionella bozemanii did not cluster with the other strains of this species, and the only strain of Legionella geestiana available clustered with the strains of Legionella feeleii. When we performed a numerical analysis by omitting certain portions of the pattern containing dense bands, all of the species could be distinguished. Our results also show that the type strains of Legionella nautarum and Legionella londiniensis deposited in the National Collection of Type Cultures do not correspond to the type strains deposited in the American Type Culture Collection. We used the results of a fatty acid and ubiquinone composition analysis to complement the SDS-PAGE results for several strains whose identities as determined by indirect immunofluorescence were doubtful. Computer-assisted SDS-PAGE of whole-cell proteins can be used in the classification of Legionella species and to identify and screen large numbers of isolates for further, in-depth taxonomic studies of smaller numbers of strains.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1099/00207713-46-1-41pt_PT
dc.identifier.issn0020-7713
dc.identifier.urihttp://hdl.handle.net/10400.26/33489
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInternational Union of Microbiological Societiespt_PT
dc.relation.publisherversionhttps://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-46-1-41pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/pt_PT
dc.subjectBacterial proteinspt_PT
dc.subjectElectrophoresis, polyacrylamide gelpt_PT
dc.subjectLegionellapt_PT
dc.subjectPhylogenypt_PT
dc.subjectReproducibility of resultspt_PT
dc.subjectNumerical analysis, computer-assistedpt_PT
dc.subjectProteínas de bactériaspt_PT
dc.subjectEletroforese em gel de poliacrilamidapt_PT
dc.subjectFilogeniapt_PT
dc.subjectReprodutibilidade dos resultadospt_PT
dc.subjectAnálise numérica assistida por computadorpt_PT
dc.titleCharacterization of Legionella species by numerical analysis of whole-cell protein electrophoresispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage49pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage41pt_PT
oaire.citation.titleInternational Journal of Systematic Bacteriologypt_PT
oaire.citation.volume46pt_PT
person.familyNameVerissimo
person.familyNameMorais
person.familyNameAlcobia Gomes
person.familyNameda Costa
person.givenNameAntonio
person.givenNamePaula Vasconcelos
person.givenNameCélia Margarida
person.givenNameMilton
person.identifier185035
person.identifier.ciencia-idD61C-E7FB-CD88
person.identifier.ciencia-idF313-B890-9B46
person.identifier.ciencia-id061F-EB4B-4A76
person.identifier.ciencia-id741D-D7E1-1A34
person.identifier.orcid0000-0003-4996-3185
person.identifier.orcid0000-0002-1939-6389
person.identifier.orcid0000-0001-6329-8527
person.identifier.orcid0000-0003-4027-4412
person.identifier.ridC-9756-2014
person.identifier.scopus-author-id6603898786
person.identifier.scopus-author-id7005946097
person.identifier.scopus-author-id7101630609
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery7f538554-a538-4c9d-8971-b66d484aafce

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