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Postmortem analyses unveil the poor efficacy of decontamination, anti-inflammatory and immunosuppressive therapies in Paraquat human intoxications

2009Journal article Open access
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dc.contributor.authorDinis-Oliveira, Ricardo Jorge
dc.contributor.authorde Pinho, Paula Guedes
dc.contributor.authorSantos, Liliana
dc.contributor.authorTeixeira, Helena
dc.contributor.authorMagalhães, Teresa
dc.contributor.authorSantos, Agostinho
dc.contributor.authorde Lourdes Bastos, Maria
dc.contributor.authorRemião, Fernando
dc.contributor.authorDuarte, José Alberto
dc.contributor.authorCarvalho, Félix
dc.date.accessioned2023-02-09T15:25:16Z
dc.date.available2023-02-09T15:25:16Z
dc.date.issued2009
dc.description.abstractBackground: Fatalities resulting from paraquat (PQ) self-poisonings represent a major burden of this herbicide. Specific therapeutic approaches have been followed to interrupt its toxic pathway, namely decontamination measures to prevent PQ absorption and to increase its excretion from organism, as well as the administration of anti-inflammatory and immunosuppressive drugs. Until now, none of the postmortem studies resulting from human PQ poisonings have assessed the relationship of these therapeutic measures with PQ toxicokinetics and related histopathological lesions, these being the aims of the present study. Methodology/principal findings: For that purpose, during 2008, we collected human fluids and tissues from five forensic autopsies following fatal PQ poisonings. PQ levels were measured by gas chromatography-ion trap mass spectrometry. Structural inflammatory lesions were evaluated by histological and immunohistochemistry analysis. The samples of cardiac blood, urine, gastric and duodenal wall, liver, lung, kidney, heart and diaphragm, showed quantifiable levels of PQ even at 6 days post-intoxication. Structural analysis showed diffused necrotic areas, intense macrophage activation and leukocyte infiltration in all analyzed tissues. By immunohistochemistry it was possible to observe a strong nuclear factor kappa-B (NF-kappaB) activation and excessive collagen deposition. Conclusions/significance: Considering the observed PQ levels in all analyzed tissues and the expressive inflammatory reaction that ultimately leads to fibrosis, we conclude that the therapeutic protocol usually performed needs to be reviewed, in order to increase the efficacy of PQ elimination from the body as well as to diminish the inflammatory process.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDinis-Oliveira RJ, de Pinho PG, Santos L, Teixeira H, Magalhães T, Santos A, de Lourdes Bastos M, Remião F, Duarte JA, Carvalho F. Postmortem analyses unveil the poor efficacy of decontamination, anti-inflammatory and immunosuppressive therapies in paraquat human intoxications. PLoS One. 2009 Sep 25;4(9):e7149pt_PT
dc.identifier.doi10.1371/journal.pone.0007149pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/43768
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationDEVELOPMENT AND APPLICATION OF ANTIDOTES FOR THE TREATMENT OF XENOBIOTIC INTOXICATIONS IN PRE-CLINICAL AND CLINICAL STUDIES USING THE MODEL OF PARAQUAT-INDUCED PULMONARY TOXICITY
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0007149pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPostmortem Analysespt_PT
dc.subjectParaquat Human Intoxicationspt_PT
dc.titlePostmortem analyses unveil the poor efficacy of decontamination, anti-inflammatory and immunosuppressive therapies in Paraquat human intoxicationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEVELOPMENT AND APPLICATION OF ANTIDOTES FOR THE TREATMENT OF XENOBIOTIC INTOXICATIONS IN PRE-CLINICAL AND CLINICAL STUDIES USING THE MODEL OF PARAQUAT-INDUCED PULMONARY TOXICITY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F36865%2F2007/PT
oaire.citation.issue9pt_PT
oaire.citation.startPagee7149pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume4pt_PT
person.familyNameTeixeira
person.familyNameSantos
person.givenNameHelena M.
person.givenNameAgostinho
person.identifier.ciencia-id4214-85EB-E58C
person.identifier.ciencia-id5311-A774-AC55
person.identifier.orcid0000-0001-7570-5521
person.identifier.orcid0000-0002-1396-4234
person.identifier.scopus-author-id7003981256
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication04b8f471-69fa-4cc1-942a-7bc7e690a0d4
relation.isAuthorOfPublication61299072-a091-458c-bb8f-8bbc062ed145
relation.isAuthorOfPublication.latestForDiscovery61299072-a091-458c-bb8f-8bbc062ed145
relation.isProjectOfPublication20ea152b-c38e-413b-92b8-4d2cea9430d7
relation.isProjectOfPublication.latestForDiscovery20ea152b-c38e-413b-92b8-4d2cea9430d7

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