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LC–MS-MS-MS3 for the determination and quantification of ∆9-tetrahydrocannabinol and metabolites in blood samples

2023Journal article Restricted access
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dc.contributor.authorProença, Paula
dc.contributor.authorTeixeira, Helena
dc.contributor.authorMartinho, Beatriz
dc.contributor.authorMonteiro, Carla
dc.contributor.authorFranco, João
dc.contributor.authorCorte-Real, Francisco
dc.date.accessioned2023-09-26T10:14:23Z
dc.date.available2023-09-26T10:14:23Z
dc.date.issued2023
dc.description.abstractDue to the high prevalence of cannabinoids in forensic toxicology analysis, it is crucial to have an efficient method that allows the use of a small sample amount and that requires a minimal sample preparation for the determination and quantification of low concentrations. A simple, highly selective and high throughput liquid chromatography–tandem mass spectrometry methodology (LC–MS-MS-MS3) was developed for the determination and quantification of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-Δ9- tetrahydrocannabinol (THCOH) and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) in blood samples. Chromatographic analysis of THC, THC-OH and THC-COOH and their deuterated internal standards was preceded by protein precipitation (PPT) of 0.1 mL of blood samples with acetonitrile. Chromatographic separation was achieved by use of an Acquity UPLC® HHS T3 (100 mm × 2.1 mm i.d., 1.8 μm) reversed-phase column, using a gradient elution of 2 mM aqueous ammonium formate, 0.1% formic acid and methanol at a flow rate of 0.4 mL/min, with a run time of 10 min. For the MS-MS-MS3 analysis, a SCIEX QTRAP® 6500+ triple quadrupole linear ion trap mass spectrometer was used via electrospray ionization (ESI), operated in multiple reaction monitoring (MRM) and linear ion trap mode (MS3). The method was validated in accordance with internationally accepted criteria and guidelines, and proved to be selective and linear between 0.5 and 100 ng/mL (r2 > 0.995). The lower limits of quantification (LLOQ) corresponded to the lowest concentrations used for the calibration curves. The coefficients of variation obtained for accuracy and precision were <15%. The mean recoveries were between 88.0% and 117.2% for the studied concentration levels (1 ng/mL, 5 ng/mL and 50 ng/mL). No significant interfering compounds, matrix effects or carryover were observed. The validated method provides a sensitive, efficient and robust procedure for the quantification of cannabinoids in blood, using LC–MS-MS-MS3 and a sample volume of 0.1 mL. This work is also a proof of concept for using LC–MS3 technique to determine drugs in biological samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationProença, P., Teixeira, H. M., Martinho, B., Monteiro, C., Franco, J., & Corte-Real, F. (2023). LC-MS-MS-MS3 for the determination and quantification of ∆9-tetrahydrocannabinol and metabolites in blood samples. Journal of analytical toxicology, 47(7), 606–614.pt_PT
dc.identifier.doi10.1093/jat/bkad046pt_PT
dc.identifier.issn1945-2403
dc.identifier.urihttp://hdl.handle.net/10400.26/46747
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relation.publisherversionhttps://academic.oup.com/jat/article/47/7/606/7231471pt_PT
dc.subjectCannabinoidspt_PT
dc.subjectLC-MS/MS-MS3pt_PT
dc.subjectWhole Bloodpt_PT
dc.subjectProtein Precipitationpt_PT
dc.titleLC–MS-MS-MS3 for the determination and quantification of ∆9-tetrahydrocannabinol and metabolites in blood samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceOxfordpt_PT
oaire.citation.endPage614pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage606pt_PT
oaire.citation.titleJournal of Analytical Toxicologypt_PT
oaire.citation.volume47pt_PT
person.familyNameProença
person.familyNameTeixeira
person.familyNameMonteiro
person.familyNameFranco
person.familyNameCorte Real Gonçalves
person.givenNamePaula
person.givenNameHelena M.
person.givenNameCarla
person.givenNameJoão
person.givenNameFrancisco
person.identifier.ciencia-id4214-85EB-E58C
person.identifier.ciencia-id0B18-918A-80ED
person.identifier.orcid0000-0002-1642-636X
person.identifier.orcid0000-0001-7570-5521
person.identifier.orcid0000-0001-6665-315X
person.identifier.orcid0000-0002-3268-3841
person.identifier.orcid0000-0003-1495-9362
person.identifier.scopus-author-id7003981256
person.identifier.scopus-author-id6603582554
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryfc1b232f-f1da-440b-9438-45a835a551af

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