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Opioid detection and quantification in plasma and oral fluid by LC-MS/MS.

2026-01-28Artigo científico Acesso aberto
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dc.contributor.authorRosendo, Luana M
dc.contributor.authorCosta, Suzel
dc.contributor.authorSimões, Susana
dc.contributor.authorFranco, João
dc.contributor.authorSerrano Gadea, Noelia
dc.contributor.authorEscorial, Mónica
dc.contributor.authorToboso Ortega, Francisco Javier
dc.contributor.authorJiménez-García, Segundo
dc.contributor.authorPeiró, Ana M
dc.contributor.authorDuque, Isabel
dc.contributor.authorRosado, Tiago
dc.contributor.authorBarroso, Mário
dc.contributor.authorGallardo, Eugenia
dc.date.accessioned2026-02-03T09:25:27Z
dc.date.available2026-02-03T09:25:27Z
dc.date.issued2026-01-28
dc.description.abstractThe opioid crisis remains a significant public health concern, necessitating the development of sensitive and reliable analytical methods for drug detection. This study aimed to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of fentanyl, buprenorphine, oxycodone, morphine, tramadol, and tapentadol in plasma and oral fluid. The method was validated according to FDA guidelines, assessing selectivity, linearity, precision, accuracy, matrix effect, extraction efficiency, stability, carryover, and dilution integrity. The lower limits of quantification (LLOQs) were established at 0.1 ng/mL for fentanyl, 1.2 ng/mL for tramadol, and 0.6 ng/mL for the remaining opioids, demonstrating high sensitivity. The method exhibited excellent precision and accuracy, with coefficients of variation below 15% for intra-day, inter-day, and intermediate precision analyses. Extraction efficiencies exceeded 90% for most analytes, and matrix effects remained within acceptable limits. Real-world application to authentic plasma and oral fluid samples confirmed the method's robustness and reliability. Oral fluid concentrations were detectable across all target opioids, although plasma-oral fluid ratios showed some compound-dependent variability. These findings highlight the potential of oral fluid as a non-invasive complementary matrix to plasma for opioid monitoring, with relevant implications for forensic toxicology and clinical drug monitoring.eng
dc.description.sponsorshipThis study was also supported by the National Drug Plan Budget from the Spanish Ministry of Health MORPHEO 2024–01), and through the UniSalut joint collaboration between Universitat Politècnica de València (UPV, POLISABIO App-DEPOP-SOFT 2024-PI1), Universidad Miguel Hernández (UMH, ILISABIO AppDEPOP 2023-AP08), and FISABIO.
dc.identifier.citationRosendo, L. M., Costa, S., Simões, S., Franco, J. M., Serrano Gadea, N., Escorial, M., Toboso Ortega, F. J., Jiménez-García, S., Peiró, A. M., Duque, I., Rosado, T., Barroso, M., & Gallardo, E. (2026). Opioid detection and quantification in plasma and oral fluid by LC-MS/MS. Analytical and bioanalytical chemistry, (2026) Advance online
dc.identifier.doi10.1007/s00216-026-06336-1
dc.identifier.issn1618-2642
dc.identifier.urihttp://hdl.handle.net/10400.26/61391
dc.language.isoen
dc.peerreviewedyes
dc.publisherSpringer
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectLC–MS/MS
dc.subjectOpioids
dc.subjectOral fluid
dc.subjectPlasma
dc.titleOpioid detection and quantification in plasma and oral fluid by LC-MS/MS.eng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.citation.titleAnalytical and bioanalytical chemistry
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCosta
person.familyNameSimões
person.familyNameFranco
person.familyNameBarroso
person.givenNameSuzel
person.givenNameSusana
person.givenNameJoão
person.givenNameMário
person.identifier.ciencia-idF611-6D58-E4C2
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0009-0002-1268-8052
person.identifier.orcid0000-0002-9457-1546
person.identifier.orcid0000-0002-3268-3841
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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