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Supercritical Fluid Extraction vs Conventional Extraction of Myrtle Leaves and Berries: Comparison of Antioxidant Activity and Identification of Bioactive Compounds

2016-07Journal article Restricted access
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dc.contributor.authorPereira, P.
dc.contributor.authorCebola, M. J.
dc.contributor.authorOliveira, M. C.
dc.contributor.authorBernardo-Gil, M. G.
dc.date.accessioned2024-10-07T15:07:16Z
dc.date.available2024-10-07T15:07:16Z
dc.date.issued2016-07
dc.description.abstractIn this work, the antioxidant capacity of extracts of Portuguese myrtle (Myrtus communis L.) is being studied over a period of three years. The samples were leaves of myrtle collected at the flowering stage and berries sampled at an early ripened stage. Supercritical fluid extraction (SFE) extracts were obtained at 23 MPa, 45 °C and a CO2 flow of 0.3 kg h−1 using ethanol as co-solvent with a flow rate of 0.09 kg h−1. Hydrodistillation was carried out in a Clevenger type apparatus and the aqueous phase was extracted with diisopropylether having obtained what is hereby designated as liquid phase extract (LPE). The antioxidant capacity of all the extracts was determined by using three different methods: the Folin–Ciocalteu, the Trolox Equivalent Antioxidant Capacity (TEAC) and the Oxygen Radical Absorbance Capacity (ORAC). The results show that the SFE extracts present a significantly higher antioxidant capacity. The extracts were characterized and quantified by HPLC-DAD-MS/MS methods. The bioactive compounds identified in all the extracts were phenolic acids (only in the LPE extracts), flavonoids and anthocyanins (only in the SFE extracts). The results indicate that the higher antioxidant capacity of the SFE myrtle extracts is mainly correlated with the concentration of flavonol glycosides, the myricetin-O-glycosides.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPereira, P., Cebola, M. J., Oliveira, M. C., & Bernardo-Gil, M. G. (2016). Supercritical fluid extraction vs conventional extraction of myrtle leaves and berries: Comparison of antioxidant activity and identification of bioactive compounds. The journal of supercritical fluids, 113, 1-9.pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.supflu.2015.09.006pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/52426
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/pt_PT
dc.subjectMyrtus communispt_PT
dc.subjectSupercritical fluid extractionpt_PT
dc.subjectEssential oilpt_PT
dc.subjectAntioxidant capacitypt_PT
dc.subjectBioactive compoundspt_PT
dc.subjectHPLC–MSpt_PT
dc.titleSupercritical Fluid Extraction vs Conventional Extraction of Myrtle Leaves and Berries: Comparison of Antioxidant Activity and Identification of Bioactive Compoundspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleThe Journal of Supercritical Fluidspt_PT
oaire.citation.volume113pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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