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Development and validation of a simple and fast method for routine analysis of new synthetic opioids and hallucinogens in whole blood using protein precipitation and UHPLC-MS/MS

2026-02Artigo científico Acesso aberto
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dc.contributor.authorPereira, Joana R.P.
dc.contributor.authorAntunes, Mónica
dc.contributor.authorNeng, N.R.
dc.contributor.authorMustra, Carla
dc.contributor.authorFranco, João
dc.contributor.authorFonseca, Suzana
dc.date.accessioned2026-04-20T14:43:56Z
dc.date.available2026-04-20T14:43:56Z
dc.date.issued2026-02
dc.description.abstractIn forensic toxicology, the rapid and reliable detection of emerging synthetic opioids and hallucinogens is crucial for case investigations and public health monitoring. This work describes the development, optimization and validation of a simple, fast and sensitive methodology for the simultaneous analysis of 6 new synthetic opioids (carfentanil, fentanyl, isotonitazene, metonitazene, norfentanyl, and sufentanil) and 2 hallucinogens (lysergide [LSD] and mescaline), together with the main LSD metabolite 2-oxo-3-hydroxy-lysergide [LSD-OH], in whole blood samples by liquid chromatography coupled to tandem mass spectrometry. Under optimized experimental conditions, linearity was verified between 0.1 and 20 ng/mL for all analytes except mescaline (2.5-500 ng/mL), with r2 > 0.99 for 1/x weighting, and no significant carryover or matrix effects were observed. Good precision (% RSD < 13 %) and trueness (% Bias within ± 20 %) values were achieved. The estimated limit of quantification (LOQ) was 0.1 ng/mL for all compounds except mescaline (2.5 ng/mL). Authentic forensic samples were also analyzed, and positive samples for fentanyl, norfentanyl, and sufentanil were identified. The proposed methodology allows the simultaneous analysis of compounds from different families of psychoactive substances, in both postmortem and in vivo samples, using only 50 µL of whole blood. The demonstrated speed, simplicity, and effectiveness make it particularly advantageous for routine implementation in forensic toxicology laboratories.eng
dc.identifier.citationPereira, J. R. P., Antunes, M., Neng, N. R., Mustra, C., Franco, J., & Fonseca, S. (2026). Development and validation of a simple and fast method for routine analysis of new synthetic opioids and hallucinogens in whole blood using protein precipitation and UHPLC-MS/MS. Forensic science international, 378, 112713.
dc.identifier.doi10.1016/j.forsciint.2025.112713
dc.identifier.issn0379-0738
dc.identifier.urihttp://hdl.handle.net/10400.26/62805
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relationCentro de Química Estrutural
dc.relationEgas Moniz Interdisciplinary Research Center
dc.relationLA/P/0056/2020
dc.relationUIDB/04585/2020
dc.relationLA/P/0056/2020
dc.relation.ispartofForensic Science International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHallucinogens
dc.subjectNew Synthetic Opioids
dc.subjectProtein Precipitation
dc.subjectUHPLC-MS/MS
dc.subjectWhole Blood
dc.titleDevelopment and validation of a simple and fast method for routine analysis of new synthetic opioids and hallucinogens in whole blood using protein precipitation and UHPLC-MS/MSeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/00100/2020
oaire.awardNumberUIDB/04585/2020
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleEgas Moniz Interdisciplinary Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00100%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04585%2F2020/PT
oaire.citation.titleForensic Science International
oaire.citation.volume378
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameSoares Antunes
person.familyNameda Rosa Neng
person.familyNameMustra
person.familyNameFranco
person.familyNameFonseca
person.givenNameMónica Sofia
person.givenNameNuno
person.givenNameCarla
person.givenNameJoão
person.givenNameSuzana
person.identifierE-4918-2012
person.identifier.ciencia-idBE1B-DDFA-21F6
person.identifier.ciencia-idD61C-FF61-87AC
person.identifier.ciencia-id5A1F-C07A-BB08
person.identifier.ciencia-idC31D-C5F1-D6BD
person.identifier.orcid0000-0002-9719-6024
person.identifier.orcid0000-0001-9879-1565
person.identifier.orcid0000-0002-4559-1735
person.identifier.orcid0000-0002-3268-3841
person.identifier.orcid0000-0001-9122-1017
person.identifier.scopus-author-id57352794100
person.identifier.scopus-author-id52464294700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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