Browsing by Author "Silva, S."
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- A novel semi-solid pill for stress-free voluntary oral drug administration in experimental rodentsPublication . Viana, Sofia; Martins, B.; Nunes, S.; Palavra, F.; Preguiça, I.; Alves, A.; Nóbrega, C.; Fernandes, R.; Silva, S.; Barbosa Moreira, Zélia; Lima, D.L.D.; Fontes-Ribeiro, Carlos; Reis, FlávioDuring compound screening and drug development, long-term oral drug administration to experimental rodents is often required. Oral gavage, a straightforward drug dosing technique, is not suitable for extended treatments considering the recurrent traumatic complications (gastroesophageal injury) and physiological distress (corticosterone levels alterations) that frequently bias experimental design outcomes. These reasons create a challenge for preclinical drug assays and stress-free/metabolic-inert alternatives of oral drug administration are warranted. Herein, it is presented an innovative semi-solid pill optimized to overcome aforementioned drawbacks. After a brief training period, C57BL/6 mice submitted to a chronic oral administration protocol (50 days) displayed a high index of voluntary acceptance of emptyand drug- (e.g. sitagliptin) incorporated vehicle in both healthy and CNS-diseased states. This protocol operates in a pair-housed animal housing fashion, allowing animal socialization throughout entire protocol. At the end of experiments, a normal neurobehavioral phenotype (anxiolytic, memory, locomotion parameters) was recorded. Moreover, this new methodology proved to be safe, preserving serum metabolic (glucose, triglycerides, total cholesterol), hepatic (albumin, total proteins) and renal (urea, creatinine, uric acid) parameters along with normal ileum contractility. Remarkably, coherent sitagliptin ( 10 mg/ml) plasma levels were detected, along with a robust decrease ( 80%) on the activity of its target (dipeptidyl peptidase- 4), unequivocally proving in vivo drug efficacy. Overall, this innovative approach may enclose a breakthrough advance for translational studies in scientific and pharmaceutical fields, providing a reproducible, efficient, metabolic inert and stress-free alternative for voluntary oral drug administration, with expected improvement on the data feasibility.
- Results of the GEP-ISFG collaborative study on an X-STR DecaplexPublication . Gusmão, L.; Alves, C.; Sánchez-Diz, P.; Zarrabeitia, M.T.; Abovich, M.A.; Aragón, I.; Arce, B.; Arrieta, G.; Arroyo, E.; Atmetlla, I.; Baeza, C.; Bobillo, M.C.; Cainé, Laura; Campos, R.; Caraballo, L.; Carvalho, E.; Carvalho, Mónica; Cicarelli, R.M.B.; Comas, D.; Corach, D.; Espinoza, M.; Espinheira, M.R.; Rendo, F.; García, O.; Gomes, I.; González, A.; Hernández, A.; Hidalgo, M.; Lozano, P.; Malaghini, M.; Manzanares, D.; Martínez, B.; Martins, J.A.; Maxzud, K.; Miguel, I.; Modesti, N.; Montesino, M.; Ortiz, R.; Pestano, J.J.; Pinheiro, M.F.; Prieto, L.; Raimondi, E.; Riancho, J.A.; Rodríguez, M.B.; Salgado, I.; Salgueiro, N.; Sánchez, J.J.; Silva, S.; Toscanini, U.; Vidales, C.; Vieira Da Silva, Cláudia; Villalobos, M.C.; Vullo, C.; Yurrebaso, I.; Zubillaga, A.I.; Carracedo, A.; Amorim, A.A collaborative work was carried out by the Spanish and Portuguese ISFG Working Group with a PCR multiplex for X chromosome STRs. Markers were selected among those described as polymorphic in humans and that have been used by some laboratories in forensics. Primers and various technical methods were investigated with the aim of optimizing a multiplex for the 10 selected X-STRs. Primer mix stock solutions were sent to the laboratories that were asked to analyse two female bloodstains, taking as reference the genetic profiles from 9947A, 9948 and NA3657 samples. In this work, we report the results obtained by 30 GEP-ISFG laboratories, using this Decaplex, as well as alternative technical conditions that also produced good results.
- The effect of progressive aerobic, resistance and stretching exercise combined with education on body weight among breast cancer survivorsPublication . Pinheiro, A.; Silva, S.; Sequeira, M.