Percorrer por autor "Botelho, Maria Filomena"
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- Cold atmospheric plasma, a novel approach against bladder cancer, with higher sensitivity for the high-grade cell linePublication . Tavares-da-Silva, Edgar; Pereira, Eurico; Pires, Ana S.; Neves, Ana R.; Braz-Guilherme, Catarina; Marques, Inês A.; Abrantes, Ana M; Gonçalves, Ana C.; Caramelo, Francisco; Silva-Teixeira, Rafael; Mendes, Fernando; Figueiredo, Arnaldo; Botelho, Maria FilomenaAntitumor therapies based on Cold Atmospheric Plasma (CAP) are an emerging medical field. In this work, we evaluated CAP effects on bladder cancer. Two bladder cancer cell lines were used, HT-1376 (stage III) and TCCSUP (stage IV). Cell proliferation assays were performed evaluating metabolic activity (MTT assay) and protein content (SRB assay). Cell viability, cell cycle, and mitochondrial membrane potential (Δψm) were assessed using flow cytometry. Reactive oxygen and nitrogen species (RONS) and reduced glutathione (GSH) were evaluated by fluorescence. The assays were carried out with different CAP exposure times. For both cell lines, we obtained a significant reduction in metabolic activity and protein content. There was a decrease in cell viability, as well as a cell cycle arrest in S phase. The Δψm was significantly reduced. There was an increase in superoxide and nitric oxide and a decrease in peroxide contents, while GSH content did not change. These results were dependent on the exposure time, with small differences for both cell lines, but overall, they were more pronounced in the TCCSUP cell line. CAP showed to have a promising antitumor effect on bladder cancer, with higher sensitivity for the high-grade cell line.
- Effect of varying functional monomers in experimental self-adhesive composites : polymerization kinetics, cell metabolism influence and sealing abilityPublication . Ferreira, Marta Nunes; Santos, Marta Neves Dos; Fernandes, Inês; Marto, Carlos Miguel; Laranjo, Mafalda; Silva, Diana; Serro, Ana Paula; Carrilho, Eunice; Botelho, Maria Filomena; Azul, Ana Mano; Delgado, António H. S.The aim was to evaluate the effects of adding different functional monomers to experimental self-adhesive composites (SACs) on polymerization kinetics, cell metabolic activity, and sealing ability. SACs were formulated using urethane dimethacrylate as the base monomer and triethylene glycol dimethacrylate. Additionally, 10 wt.% of distinct functional monomers were added - 10-methacryloyloxydecyl dihydrogen phosphate, glycerol phosphate dimethacrylate (GPDM), 2-hydroxyethyl methacrylate (HEMA) or hydroxyethyl acrylamide (HEAA). ATR-FTIR was used to determine real-time polymerization kinetics (20 min, n = 3). The final extrapolated conversion and polymerization rates were determined (DC,max;Rp,max). The DC,max values were employed to calculate volumetric shrinkage. The MTT assay was performed on MDPC-23 cells using disc extracts at different concentrations (n = 8). Class V cavities were prepared in 60 sound human molars, assigned to six groups (n = 10), depending on the composite used and aging type (T0 or TC, if thermocycled for 10 000 cycles). One-way ANOVA, two-way, and Kruskal–Wallis tests were employed to treat the data (ɑ = 0.05). Varying the functional monomers had a large impact on DC,max, as confirmed by one-way ANOVA (p<0.001). The highest was obtained for HEMA (64 ± 3%). The HEMA and HEAA formulations were found to be significantly more toxic at concentrations below 100%. For microleakage, having a functional monomer or not did not show any improvement, irrespective of margin or aging period (Mann–Whitney U, p > 0.05). Larger functional monomers MDP and GPDM affected polymerization properties. Conversely, their acidity did not seem to be detrimental to cell metabolic activity. Regarding sealing ability, it seems that the functional monomers did not bring an advantage to the composites. Varying the functional monomer in SACs had a clear impact on the polymerization kinetics as well as on their cytotoxic potential. However, it did not confer better microleakage and sealing. Claiming self-adhesiveness based only on functional monomers seems dubious.
- Portal venous pressure variation during hepatectomy: a prospective studyPublication . Carrapita, Jorge Gomes; Rocha, Clara; Donato, Henrique; Costa, Alexandre; Abrantes, Ana Margarida; Santos, Jorge Nunes; Botelho, Maria Filomena; Tralhão, José Guilherme; Barbosa, Jorge MacielExcessive portal venous pressure in the liver remnant is an independent factor in the occurrence of posthepatectomy liver failure and small-for-size syndrome. The baseline portal pressure prior to hepatectomy was not considered previously. The aim of this study is to assess the impact of portal pressure change during hepatectomy on the patient outcome.