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Methylation analysis of Klebsiella pneumoniae from Portuguese hospitals

2021-03Contribution to journal Open access
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datacite.subject.fosCiências Médicas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorSpadar, Anton
dc.contributor.authorPerdigão, João
dc.contributor.authorPhelan, Jody
dc.contributor.authorCharleston, James
dc.contributor.authorModesto, Ana
dc.contributor.authorElias, Rita
dc.contributor.authorSessions, Paola Florez de
dc.contributor.authorHibberd, Martin L.
dc.contributor.authorCampino, Susana
dc.contributor.authorDuarte, Aida
dc.contributor.authorClark, Taane G.
dc.date.accessioned2025-10-08T11:11:05Z
dc.date.available2025-10-08T11:11:05Z
dc.date.issued2021-03
dc.description.abstractKlebsiella pneumoniae is an important nosocomial infectious agent with a high antimicrobial resistance (AMR) burden. The application of long read sequencing technologies is providing insights into bacterial chromosomal and putative extra-chromosomal genetic elements (PEGEs) associated with AMR, but also epigenetic DNA methylation, which is thought to play a role in cleavage of foreign DNA and expression regulation. Here, we apply the PacBio sequencing platform to eight Portuguese hospital isolates, including one carbapenemase producing isolate, to identify methylation motifs. The resulting assembled chromosomes were between 5.2 and 5.5Mbp in length, and twenty-six PEGEs were found. Four of our eight samples carry blaCTX-M-15, a dominant Extended Spectrum Beta Lactamase in Europe. We identified methylation motifs that control Restriction–Modification systems, including GATC of the DNA adenine methylase (Dam), which methylates N6-methyladenine (m6A) across all our K. pneumoniae assemblies. There was a consistent lack of methylation by Dam of the GATC motif downstream of two genes: fosA, a locus associated with low level fosfomycin resistance, and tnpB transposase on IncFIB(K) plasmids. Overall, we have constructed eight high quality reference genomes of K. pneumoniae, with insights into horizontal gene transfer and methylation m6A motifs.eng
dc.identifier.citationSpadar, A., Perdigão, J., Phelan, J. et al. Methylation analysis of Klebsiella pneumoniae from Portuguese hospitals. Sci Rep 11, 6491 (2021). https://doi.org/10.1038/s41598-021-85724-2
dc.identifier.doi10.1038/s41598-021-85724-2
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.26/58957
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Nature
dc.relation.hasversionhttps://doi.org/10.1038/s41598-021-85724-2
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectKlebsiella pneumoniae
dc.subjectMethylation
dc.subjectHospitals
dc.subjectPortugal
dc.titleMethylation analysis of Klebsiella pneumoniae from Portuguese hospitalseng
dc.typecontribution to journal
dspace.entity.typePublication
oaire.citation.startPage6491
oaire.citation.titleScientific Reports
oaire.citation.volume11
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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