Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage

Fructophily is a rare trait that consists of the preference for fructose over other carbon sources. Here, we show that in a yeast lineage (the Wickerhamiella/Starmerella, W/S clade) comprised of fructophilic species thriving in the high-sugar floral niche, the acquisition of fructophily is concurrent with a wider remodeling of central carbon metabolism. Coupling comparative genomics with biochemical and genetic approaches, we gathered ample evidence for the loss of alcoholic fermentation in an ancestor of the W/S clade and subsequent reinstatement through either horizontal acquisition of homologous bacterial genes or modification of a pre-existing yeast gene. An enzyme required for sucrose assimilation was also acquired from bacteria, suggesting that the genetic novelties identified in the W/S clade may be related to adaptation to the high-sugar environment. This work shows how even central carbon metabolism can be remodeled by a surge of HGT events.

Palavras-chave

Tarmerella Alcoholic fermentation Evolutionary biology Floral niche Fructophilic yeasts Genomics Horizontal gene transfer Sugar metabolism

URI

http://hdl.handle.net/10400.26/30510

Citação

Gonçalves et al. eLife 2018;7:e33034. DOI: https://doi.org/10.7554/eLife.33034

Editora

eLife Sciences Publications

DOI

10.7554/eLife.33034

Coleções

EM - Artigos Científicos

Licença CC

cclicense-by

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