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Determination of amphetamine-type psychostimulants in hair samples using MEPS as sample clean-up and gas-chromatography coupled with mass spectrometry

2023-12Other Open access
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dc.contributor.authorPires, Bruno
dc.contributor.authorSimão, Ana Y.
dc.contributor.authorRosado, Tiago
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorBarroso, Mário
dc.date.accessioned2024-01-24T12:45:00Z
dc.date.available2024-01-24T12:45:00Z
dc.date.issued2023-12
dc.descriptionComunicação apresentada no 13º Encontro Nacional de Cromatografiapt_PT
dc.description.abstractNumerous protocols for the analysis of amphetamine-type psychostimulants (ATS) in hair have been employed over the years. Microextraction by packed sorbent (MEPS), a downsized version of solid-phase extraction (SPE), has been effectively applied in hair drug analysis, including substances like opiates, cocaine, and ketamine. However, concerning ATS, MEPS has primarily been used to the determination of amphetamine (AMP) and methamphetamine (MAMP) in hair1-4. The main objective of this study was to develop and validate a method using MEPS as a sample clean-up, for the determination of AMP, MAMP, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 1-(1,3-benzodioxol-5-yl)propan-2-yl(ethyl)amine (MDE), and N-methyl-1-(1,3-benzodioxol-5-yl)-2-aminobutane (MBDB) in hair. The extraction procedure involved incubating 50 mg of hair with 1M NaOH at 45 ºC overnight, followed by neutralization with 10M HCl and centrifugation5. A Design of Experiments (DoE) approach was employed to optimize MEPS clean-up process, encompassing conditioning, loading, and elution steps. The eluted extract underwent derivatization and was analysed using gas chromatography coupled to mass spectrometry (GC-MS). The developed MEPS method yielded recoveries ranging from 8 to 52% for the different analytes in hair samples and linearity was obtained between 0.2 (cut-off proposed by SoHT) and 5.0 ng/mg. The precision and accuracy were in accordance to international method validation standards. This study introduces the first analytical method integrating MEPS with GC-MS for the detection of these specific amphetamines in hair samples. Notably, this method offers a viable alternative to conventional procedures, characterized by its speed, eco-friendliness, and cost-effectiveness.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/49323
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationHair as a monitoring tool for new drugs: synthetic cannabinoids and opioids
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnfetaminaspt_PT
dc.subjectCabelopt_PT
dc.subjectMEPSpt_PT
dc.subjectAmphetaminept_PT
dc.subjectHairpt_PT
dc.titleDetermination of amphetamine-type psychostimulants in hair samples using MEPS as sample clean-up and gas-chromatography coupled with mass spectrometrypt_PT
dc.typeother
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHair as a monitoring tool for new drugs: synthetic cannabinoids and opioids
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PDQI_CENTRO/2020.09070.BD/PT
oaire.citation.conferencePlaceLisboapt_PT
oaire.citation.title13º Encontro Nacional de Cromatografiapt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPDQI_CENTRO
person.familyNameBarroso
person.givenNameMário
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeotherpt_PT
relation.isAuthorOfPublication294b555e-48c4-4013-b981-b8917400c56c
relation.isAuthorOfPublication.latestForDiscovery294b555e-48c4-4013-b981-b8917400c56c
relation.isProjectOfPublication4ca3c3c9-1802-4c7f-8730-9547236896fe
relation.isProjectOfPublication56ce2e5c-35e5-4eb7-8152-2e87e77f638c
relation.isProjectOfPublicatione951cc6d-0466-4e2e-b4d9-7dd14ba7a196
relation.isProjectOfPublication.latestForDiscovery4ca3c3c9-1802-4c7f-8730-9547236896fe

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