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As metaloproteinases como biomarcadores da doença periodontal : estudo piloto
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Abstract(s)
Objetivo: Avaliar a presença de MMP ativas no FCG de pacientes com doença periodontal - grupo de estudo - por comparação a pacientes com saúde periodontal - grupo de controlo -, a fim de determinar se estas enzimas podem atuar como biomarcadores da doença.
Metodologia: Participaram neste estudo cinco pacientes com saúde periodontal e cinco pacientes com periodontite. O fluido foi recolhido por Periopapers em sulcos gengivais e bolsas periodontais e quantificado no Periotron. A presença de MMP ativas nas amostras de FCG foi analisada por zimografia de colagénio.
Resultados: O volume de FCG foi superior em bolsas periodontais (154 ± 38 (n=8)) do que em sulcos gengivais (26 ± 17 (n=10)). A incubação dos géis por um período de tempo alargado permitiu detetar um maior número de bandas proteolíticas previsivelmente correspondentes a formas enzimáticas inativas (pró-MMP-8/pró-MMP-13) e ativas (MMP-1/MMP-13 e MMP-2), tendo sido observadas diferenças entre os grupos estudados.
Conclusão: A utilização de Periopapers e do Periotron constituiu um método adequado de recolha e quantificação de FCG. A atividade proteolítica das MMP apresentou diferenças entre os dois grupos estudados, o que revela que estas enzimas podem ser indicadas como biomarcadores da doença.
Objective: To evaluate the presence of active MMPs in the GCF of patients with periodontal disease - study group - in comparison with patients with periodontal health - control group -, in order to determine whether these enzymes can act as biomarkers of the disease. Methodology: Five patients with periodontal health and five patients with periodontitis participated in this study. The fluid was collected by Periopapers in gingival sulcus and periodontal pockets and quantified in Periotron. The presence of active MMPs in GCF samples was analyzed by collagen zymography. Results: The volume of GCF was higher in periodontal pockets (154 ± 38 (n=8)) than in gingival sulcus (26 ± 17 (n=10)). The incubation of the gels for an extended period of time allowed to detect a greater number of proteolytic bands predictably corresponding to inactive (pro-MMP-8/pro-MMP-13) and active (MMP-1/MMP-13 and MMP-2) enzymatic forms, with differences between the studied groups being observed. Conclusion: The use of Periopapers and Periotron was an adequate method for collecting and quantifying GCF. The proteolytic activity of MMPs showed differences between the two groups studied, which reveals that these enzymes can be indicated as biomarkers of the disease.
Objective: To evaluate the presence of active MMPs in the GCF of patients with periodontal disease - study group - in comparison with patients with periodontal health - control group -, in order to determine whether these enzymes can act as biomarkers of the disease. Methodology: Five patients with periodontal health and five patients with periodontitis participated in this study. The fluid was collected by Periopapers in gingival sulcus and periodontal pockets and quantified in Periotron. The presence of active MMPs in GCF samples was analyzed by collagen zymography. Results: The volume of GCF was higher in periodontal pockets (154 ± 38 (n=8)) than in gingival sulcus (26 ± 17 (n=10)). The incubation of the gels for an extended period of time allowed to detect a greater number of proteolytic bands predictably corresponding to inactive (pro-MMP-8/pro-MMP-13) and active (MMP-1/MMP-13 and MMP-2) enzymatic forms, with differences between the studied groups being observed. Conclusion: The use of Periopapers and Periotron was an adequate method for collecting and quantifying GCF. The proteolytic activity of MMPs showed differences between the two groups studied, which reveals that these enzymes can be indicated as biomarkers of the disease.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Doença periodontal Fluido crevicular gengival Metaloproteinases Biomarcadores