Publication
Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort
| dc.contributor.author | Brandão, Ruben | |
| dc.contributor.author | Marcelino, Rute | |
| dc.contributor.author | Gonçalves, Fátima | |
| dc.contributor.author | Diogo, Isabel | |
| dc.contributor.author | Carvalho, Ana | |
| dc.contributor.author | Cabanas, Joaquim | |
| dc.contributor.author | Costa, Inês | |
| dc.contributor.author | Brogueira, Pedro | |
| dc.contributor.author | Ventura, Fernando | |
| dc.contributor.author | Miranda, Ana | |
| dc.contributor.author | Mansinho, Kamal | |
| dc.contributor.author | Gomes, Perpétua | |
| dc.date.accessioned | 2019-12-20T16:14:10Z | |
| dc.date.available | 2019-12-20T16:14:10Z | |
| dc.date.issued | 2018-04 | |
| dc.description | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | pt_PT |
| dc.description.abstract | This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all GT1-infected patients with 9.9% (8/81) having the Y93C/H mutation. NS5B RASs showed a prevalence of 14.8% (12/81) and were only detected in GT1b. Overall 38.3% (31/81) of all GT1 HCV-infected patients presented baseline RASs. The obtained data supports the usefulness of resistance testing prior to treatment since a statistically significant association was found between treatment failure and the baseline presence of specific NS5 RASs known as Y93C/H (p = 0.04). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Brandão, R.; Marcelino, R.; Gonçalves, F.; Diogo, I.; Carvalho, A.; Cabanas, J.; Costa, I.; Brogueira, P.; Ventura, F.; Miranda, A.; Mansinho, K.; Gomes, P. Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort. Viruses 2018, 10, 223. | pt_PT |
| dc.identifier.doi | 10.3390/v10050223 | pt_PT |
| dc.identifier.issn | 1999-4915 | |
| dc.identifier.uri | http://hdl.handle.net/10400.26/30657 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation.publisherversion | https://doi.org/10.3390/v10050223 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Hepatitis C virus | pt_PT |
| dc.subject | Direct-acting antivirals | pt_PT |
| dc.subject | Resistance-associated substitutions | pt_PT |
| dc.subject | NS5A | pt_PT |
| dc.subject | NS5B | pt_PT |
| dc.title | Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | E223 | pt_PT |
| oaire.citation.title | Viruses | pt_PT |
| oaire.citation.volume | 10(5) | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |