Please use this identifier to cite or link to this item:
Title: Candida clinical species identification: molecular and biochemical methods
Author: Costa, AR
Silva, F
Henriques, M
Azeredo, J
Oliveira, R
Faustino, A
Keywords: Candida
Issue Date: 2010
Publisher: Springer
Citation: Ann Microbiol. 2010; 60:105-12
Abstract: In the last decade, the number and diversity of nosocomial Candida infections has increased significantly, resulting in an emergent need for rapid and accurate methods for Candida identification. Therefore, the aim of this study was to evaluate the performance of three biochemical systems (Auxacolor, ID32C, and Vitek 2 YST) for the identification of Candida species, comparing them with molecular identification (polymerase chain reaction and gel agarose electrophoresis). These methods were used to assess Candida spp. (229 clinical isolates) prevalence and distribution among clinical specimens. The biochemical methods with higher percentages of correct identification were Vitek 2 YST (79.6%) and Auxacolor (78.6%). However, overall the biochemical methods assayed differed from the molecular identification. Thus, due to their rapid and precise identification, molecular methods are promising techniques for Candida species identification in clinical laboratories. Candida albicans and Non Candida albicans Candida species had a similar prevalence (50.4 and 49.6%, respectively), corroborating the epidemiological shift observed for these pathogens in the recent years.
Peer review: yes
Appears in Collections:HB - PAT CLIN - Artigos

Files in This Item:
File Description SizeFormat 
Candida clinical species identification.pdf170,42 kBAdobe PDFView/Open

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.