Raposo, Rodrigo S.Almeida, M. Catarina M. D. deFonseca, M. Manuela daCesário, M. Teresa2019-12-092019-12-092017-01R.S. Raposo et al. /New Biotechnology, 2017 Jan 25;34:12-22. doi: 10.1016/j.nbt.2016.10.0011871-6784http://hdl.handle.net/10400.26/30413"Efficient production of poly-3-hydroxybutyrate (P(3HB)) based on glucose-xylose mixtures simulating different types of lignocellulosic hydrolysate (LCH) was addressed using Burkholderia sacchari, a wild strain capable of metabolizing both sugars and producing P(3HB). Carbon catabolite repression was avoided by maintaining glucose concentration below 10g/L. Xylose concentrations above 30g/L were inhibitory for growth and production. In fed-batch cultivations, pulse size and feed addition rate were controlled in order to reach high productivities and efficient sugar consumptions. High xylose uptake and P(3HB) productivity were attained with glucose-rich mixtures (glucose/xylose ratio in the feed=1.5w/w) using high feeding rates, while with xylose-richer feeds (glucose/xylose=0.8w/w), a lower feeding rate is a robust strategy to avoid xylose build-up in the medium. Xylitol production was observed with xylose concentrations in the medium above 30-40g/L. With sugar mixtures featuring even lower glucose/xylose ratios, i.e. xylose-richer feeds (glucose/xylose=0.5), xylonic acid (a second byproduct) was produced. This is the first report of the ability of Burkholderia sacchari to produce both xylitol and xylonic acid."engGlucose/xylose mixturesBurkholderia sacchariXylose consumptionoly-3-hydroxybutyrateXylitolXylonic acidjournal article10.1016/j.nbt.2016.10.001