Pereira, Gonçalo MartinsPereira, Sofia S.Santos, MadalenaBrito, JoséFreitas, DiamantinoCarvalho, António Oliveira deÁguas, ArturOliveira, Maria JoãoOliveira, Pedro2025-07-212025-07-212020-07Martins Pereira G, Pereira SS, Santos M, Brito J, Freitas D, Oliveira de Carvalho A, Águas A, Oliveira MJ, Oliveira P. Effects of high-intensity infrasound on liver lipid content of rats. Heliyon. 2020 Jul 4;6(7):e04383. doi: 10.1016/j.heliyon.2020.e043832405-8440http://hdl.handle.net/10400.26/58141Previous experimental studies show that exposure to noise with high and audible frequencies causes multiple metabolic alterations, such as increased liver glycogen and triglycerides. However, the effect of exposure to sound with lower frequencies, such as high-intensity infrasound (frequency <20 Hz and sound pressure level >90 dB), on the liver lipid content is still unclear. As such, we aimed to study the effect of exposure to high-intensity infrasound of both normal and glucose intolerant rats on the liver lipid content. For this study, 79 wild-type male Wistar rats were randomly divided into two groups: G1, no treatment, and G2, induced glucose intolerance. Each of these two groups was randomly divided in two subgroups: s (animals kept in silence) and i (animals continuously exposed to high-intensity infrasound noise). At three noise-exposure time-points (1, 6 and 12 weeks) the rats were sacrificed, the liver was excised and hepatic lipids extracted. Data analysis was performed using a two-way ANOVA (p = 0.05). No significant effects due to interactions between the several factors exist on the liver lipid content (p=0.077). Moreover, no significant effects due to infrasound exposure (p=0.407) or glucose tolerance status (p=0.938) were observed. Our study shows that continuous exposure to high-intensity infrasound has no influence on the lipid content of the liver of both normal and glucose intolerant animals. This finding reinforces the need for further experimental studies on the physiological effects of infrasound due to its possible hazardous effects on human health.engInfrasoundGlucose intoleranceLiver lipidsLiver steatosisRatEnvironmental healthDiabetesObesityHepatobiliary systemEndocrine systemMetabolic disorderEndocrinologycontribution to journal10.1016/j.heliyon.2020.e04383